Exercise 1: Cell Transport Mechanisms and Permeability Worksheet Student Name: Student ID# Student instructions: Follow the step-by-step instructions for this exercise found in your lab manual and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Rename this document to include your first and last name prior to submitting‚ e.g. Exercise1_JohnSmith.doc. Please make sure that your answers are typed in RED. Grading:
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PHYSIO EX 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. The temperature of the solutions and the difference in concentration of the ion or molecule between the sides of the membrane 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Since the chemical formula for urea is (NH2)2CO and its molecular weight is about 60. It will not diffuse through the
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Paige Wicks-Niehaus A & P II Review Sheet 38 (1-7‚ 9-17‚ 19‚ 20) 10/02/2011 1. Mucosa – epithelium‚ lamina propria‚ muscularis mucosae; secretion‚ absorption‚ protection Submucosa – nutrition‚ protection Muscularis externa – regulates GI motility Serosa or Adventitia – visceral peritoneum; reduce friction‚ anchor/protect organs 2. Alimentary‚ digestive 3. It can expand and contract; number of layers 4. Replaced by smooth muscle 5. Large intestine breaks stuff down‚ colon
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Activity 1 Pre-Lab Quiz K+ inside a cell = 150 mM; K+ outside a cell = 5 mM Na+ inside a cell = 5 mM; Na+ outside a cell = 150 mM Stop & Think question What is the polarity of the resting membrane potential (voltage)? Negative What does it mean that the voltage just inside the membrane is negative? B Membrane of most cells contains K+ leak channels…..b What will happen to the resting membrane potential if the extracellular K+ concentration is increase? Less negative What happens
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Physioex 8.0 R E V I E W S H E E T E X E R C I S E 10 Acid-Base Balance Respiratory Acidosis and Alkalosis The following questions refer to Activity 1: Normal Breathing. 1. What was the pH level during normal breathing? 2. Was this pH within the normal pH range? 2. Was this pH within the normal pH range? The following questions refer to Activity 2: Hyperventilation. 3. In run 1‚ what was the maximum pH recorded with hyperventilation? 4. What acid-base imbalance occurred with hyperventilation
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Activity 1: Electrical Stimulation 1. Do you see any kind of response on the oscilloscope screen? NO 2. What was the threshold voltage‚ or the voltage at which you first saw an action potential? 3.0 V 3. How does this tracing compare to the one that was generated at the threshold voltage? The tracing travel in the same fashion but the tracing were different with an increase threshold voltage 4. What reason can you give for the change? Increase voltage 5. Record this maximal voltage here:
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Activity 1 1. Increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels because the membrane is permeable to K+ ions. Therefore‚ the K+ ions will diffuse down its concentration gradient from a region of higher concentration to a region of lower concentration. 2. Increasing extracellular K+ causes the membrane potential to change to a less negative value because the K+ ions diffuse out across the membrane. My results went well compared to my prediction
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Activity FOUR There is a direct relationship between the MWCO and the filtration rate; the higher the MWCO‚ the higher the filtration rate. Powdered charcoal is the solute that did not appear in the filtrate when using any MWCO membranes. As pressure increases‚ the filtration rate also increases‚ as long as the MWCO does not decrease. Since we have no control over the MWCO of a living membrane‚ we can increase the rate of filtration by increasing the pressure. The molecular weight
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M09_ZAO2177_01_SE_CH09.QXD 3/4/11 2:08 AM Page 143 R E V I E W S H E E T EXERCISE NAME ________________________________ LAB TIME/DATE _______________________ A C T I V I T Y 1 9 Renal System Physiology The Effect of Arteriole Radius on Glomerular Filtration 1. What are two primary functions of the kidney? ____________________________________________________________ __________________________________________________________________________________________________ 2. What are the components
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Electrical Stimulation Activity 1: Direct Heart Stimulation 1. Did you see any change in the trace? No change in the trace 2. Why or why not? This is because it needs more simulation 3. Did you see any change in the trace? I did see a change in the trace 4. Why or why not? There is double stimulation 5. Describe the change you see in the trace. How does it differ from the baseline trace? The trace increased from the baseline trace 6. What effect do the repeated stimuli
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