instructions for this exercise found in your lab manual and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Rename this document to include your first and last name prior to submitting‚ e.g. Exercise2_JohnSmith.doc. Please make sure that your answers are typed in RED. (You may delete these instructions before submission.) Grading: True/False‚ Multiple-Choice‚ and Fill-in-the-blank type questions will be worth 1 point each whereas
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PhysioEX notes Autorhythmicity- heart’s ability to trigger its own contractions Phase O- a lot like depolarization in neuronal action potentials. Sodium channels open‚ increase of sodium INTO cell. Phase 1- sodium channels close‚ potassium channels close‚ decrease in potassium and sodium. Calcium channels open‚ increase of calcium into cell. Phase 2- Plateau phase‚ membrane still depolarized (contract). Potassium channels closed‚ L-type calcium channels stay open. Lasts 0.2 seconds/200
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Activity 1 1. Increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels because the membrane is permeable to K+ ions. Therefore‚ the K+ ions will diffuse down its concentration gradient from a region of higher concentration to a region of lower concentration. 2. Increasing extracellular K+ causes the membrane potential to change to a less negative value because the K+ ions diffuse out across the membrane. My results went well compared to my prediction
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EXERCISE 10: Acid-Base Balance Grading: True/False‚ Multiple-Choice‚ and Fill-in-the-blank type questions will be worth 1 point each whereas Short-answer type questions will be worth 2 points each. This lab will be worth a total of 45 points but will be converted to a percentage grade when registered in your “Gradebook”. |ACTIVITY 1: Hyperventilation |Answers | |A
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Activity 1 Pre-Lab Quiz K+ inside a cell = 150 mM; K+ outside a cell = 5 mM Na+ inside a cell = 5 mM; Na+ outside a cell = 150 mM Stop & Think question What is the polarity of the resting membrane potential (voltage)? Negative What does it mean that the voltage just inside the membrane is negative? B Membrane of most cells contains K+ leak channels…..b What will happen to the resting membrane potential if the extracellular K+ concentration is increase? Less negative What happens
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Electrical Stimulation Activity 1: Direct Heart Stimulation 1. Did you see any change in the trace? No change in the trace 2. Why or why not? This is because it needs more simulation 3. Did you see any change in the trace? I did see a change in the trace 4. Why or why not? There is double stimulation 5. Describe the change you see in the trace. How does it differ from the baseline trace? The trace increased from the baseline trace 6. What effect do the repeated stimuli
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PhysioEx – Exercise 9 Activity 1: 1. excretion and regulation 2. glomerular capillaries (glomerulus) & Bowman’s capsule 3. The filtrate flows from the Bowman’s capsule into the renal tubule called the proximal convoluted tubule then into the loop of Henle‚ and finally into the distal convoluted tubule: a. Proximal Convoluted Tubule b. Loop of Henle c. Distal Convoluted Tubule 4. When the radius of the afferent arteriole was decreased‚ the pressure and the filtration rate both decreased
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Alkalosis Activity 1: Normal Breathing 1. At 20 seconds‚ pH = 7.4 2. At 40 seconds‚ pH = 7.4 3. At 60 seconds‚ pH = 7.4 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? No‚ it stayed at 7.4 5. Was the pH level always within the “normal” range for the human body? Yes 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No‚ it stayed at 40mm Hg Activity 2a: Hyperventilation – Run 1 1. At 20 seconds‚ pH
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Activity 1: Normal Breathing 1. At 20 seconds‚ pH = 7.4 2. At 40 seconds‚ pH = 7.4 3. At 60 seconds‚ pH = 7.4 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? The pH did not change during the normal breathing. 5. Was the pH level always within the “normal” range for the human body? Yes it was. 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No it did not change either. Activity 2a: Hyperventilation – Run 1 1. At 20
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Activity 1: Electrical Stimulation 1. Do you see any kind of response on the oscilloscope screen? NO 2. What was the threshold voltage‚ or the voltage at which you first saw an action potential? 3.0 V 3. How does this tracing compare to the one that was generated at the threshold voltage? The tracing travel in the same fashion but the tracing were different with an increase threshold voltage 4. What reason can you give for the change? Increase voltage 5. Record this maximal voltage here:
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