A Quantitative Enzyme Study: CATALASE FlowChart Purpose: Measure the rate of enzyme activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9
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Introduction Enzymes are protein based structures that help speed up chemical reactions. They help these reactions keep up with the everyday metabolic needs and other like functions of organisms. Enzymes are also considered catalysts‚ due to the lowering in activation energy‚ in which they are not consumed or changed at any point during the reaction. These enzymes have three main protein structures that help keep them formed and intact. Stage 1 of these structures is the primary structure‚ which
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The role of protein in hormone enzyme functions Proteins are very important particles in our cells. Proteins are included in basically all bodily cell functions‚ including cellular regeneration and repair‚ tissue maintenance and regulation‚ hormone and enzyme production (James‚ C.S. Delores). They vary in structure as well as function. Talking about hormone and enzyme production‚ it is important to understand that amino acids are the basic components of hormones‚ which are critical chemical
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Jolly Nguyen 11C Conditions needed for effective enzyme action Aim: To investigate the activity of enzymes and how might the activity be effected in different conditions. Hypothesis: Temperature rises and change of substrate concentration may cause denaturation of the protein of enzymes. So as the temperature rises the amount of active enzyme progressively decreases‚ and the rate is slowed. Exposure to heat causes atoms to vibrate violently and this disrupts bonds within globular proteins
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–Anatomy and Physiology Winter 2015 Class meeting: Mieklejohn‚ Rm. 2002‚ MW 8:00-‐ 9:50 Instructor: Dr. Tess Freidenburg Office: Science N403 Office hours: Wednesdays‚ 11:00am – 1:00pm‚ or by appointment Email: tess.freidenburg@csueastbay.edu (this is the best way to reach me!) Required materials: • • • Human Anatomy and Physiology‚ Marieb and
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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Enzyme Reaction Rates Under Different Conditions Introduction In this lab you will observe how the rate of a reaction is changed as certain factors are changed. You will observe how changing factors like temperature‚ pH‚ substrate concentration‚ and enzyme concentration changes the rate of an enzymatic reaction. In this experiment you will act as an enzyme by breaking toothpicks witch act as substrates. After observing the results of this experiment you will be able to determine what causes the
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- LABORATORY REPORT Activity 8: Respiratory Volumes Name: Instructor: Date: PREDICTIONS 1. During exercise: 2. During exercise: 3. During exercise: 4. During exercise: 5. During exercise: MATERIALS AND METHODS 1. Dependent Variable. 2. Independent Variable. 3. Controlled Variables. 4. Which respiratory volume was calculated? 5. What was the purpose of the nose clip? RESULTS See Table 2: Average Breathing Rates and Lung Volumes See
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