Methods for studying cells in the lab Centrifugation A centrifuge is a device for separating particles from a solution according to their size‚ shape‚ density‚ viscosity of the medium and rotor speed. Centrifugation can be used to separate cells from one culture or to isolate an organelle from the rest of the cell. The process relies on speed; the faster and longer the particle is centrifuged‚ the smaller the particles are that will be separated. Separation occurs by causing the larger particles
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treatment of a pH shock and neutralization‚ by depicting a constant percentage of immobile cells. Prior to the lab series‚ it was expected that the positive control would present a decreasing percentage of immobile cells‚ and the negative control would present an increasing percentage of immobile cells. The experimental groups‚ testing the hypothesis‚ was inferred to show a constant number of immobile cells‚ after the 60-minute light treatment. Colchicine is a substance that reduces inflammation
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John Leone Lab Partner(s) Ethan Davis & Ryan Hanna Date: 12/10/14 Instructor: Dr. Chuckran Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain
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and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Globin: 4 folded polypeptide chains * Heme: Iron group in each polypeptide that binds 02 Enzymes: * Glycolytic: generate energy through glycolysis due to lack of mitochondria * Carbonic anhydrase: for transport of CO2 into HCO3 Erythropoiesis: * regeneration of new cells in the bone marrow Erythropoietin (hormone): * when kidneys detect not enough 02 they secrete this hormone which stimulates erythropoiesis‚ increasing 02 capacity Reticulocytes: * immature RBC’s released
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Lab design –Voltaic Cells Aim Of Experiment In this experiment‚ we are going to make a battery by making an electron current between the metals Zinc and Copper. We aim two study the voltaic cells and the transfer of electrons from the metal to the ion. The tendency for the electrons to flow from one chemical to another‚ such as from the zinc metal to the copper ion as shown here‚ is something that can be channeled and controlled. Channeling the flow of electrons is what we will take up in this
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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differentiation are fundamental stem cell fate decisions‚ which are essential for normal tissue development‚ homeostasis‚ and repair actin cytoskeleton and Rho-GTPases‚ mediate downstream signal transduction Serum response factor (SRF) and yes-associated protein (YAP) are two examples of mechano-sensitive transcription factors‚ which have recently been implicated in epidermal and mesenchymal stem cell differentiation Lab notes: Passive processes: transport driven by concentration ‚ or pressure
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citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. (May 2013) As in other cells‚ the cardiac action potential is a short-lasting event in which the difference of potential between the interior and the exterior of each cardiac cell rises and falls following a consistent trajectory.[1] The cardiac action potential differs significantly in different portions of the heart. The heart is provided with a
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Objectives: To study the effects of hypotonic‚ hypertonic and isotonic solutions on plant and animal cells. Materials and Apparatus: * Microscope * Electronic balance * Stirring rod * Small knife * Glass slide and cover * 250‚ 500 ml beakers * Distilled water * Cotton * Test tubes * Spatula * Onion * Ethanol (70%) * Filter paper * Table sugar (sucrose) * Table salt (NaCl) * Potato EXPERIMENT 1 PART 1 Procedure: 1. The epidermal
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