Group No. 10 Date Started: June 11‚ 2013 Tongco‚ Val Justin DM. Date Finished: June 18‚ 2013 Santos‚ Erickson S. Vasquez‚ Jona Candace P. Wong‚ Victor Lorenzo E. Experiment 1 Use of Micropipettor and Spectrophotometer Introduction In scientific experiments‚ correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a & b) are constants. In the equation‚ A = abc where A is the absorbance‚ a is the specific absorbance‚ b is the
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The Effect of Concentration on Absorbance Background Information The purpose of the “Determining Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration
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INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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BEETROOT PIGMENTS - and membranes – These pigments are betalain pigments (not‚ as often thought‚ anthocyanins)‚ which they replace in some organisms. They are named after the Beet family of plants (Beta) but are also found in fungi (Fly Agaric - the red‚ spotted one!). In petals they presumably attract pollinating insects and may be present in seeds/fruits to encourage birds to eat them and so disperse the seeds. Man has selected for colour in beetroot‚ both because
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Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A spectrophotometer can be used quantitatively
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REPORT NAME :princess cucumber TOPIC : 3.8 & 8.2 : PHOTOSYNTHETIC PIGMENTS RESEARCH QUESTION INTRODUCTION The photosynthetic pigments are responsible for absorbing and trapping light energy the major pigments of photosynthesis are the chlorophylls. The two chlorophylls found in green plants are chlorophyll a and chlorophyll b. other pigments include carotenoids and phycobilins‚ sometimes referred to as accessory pigments. Carotenoids occur in all photosynthetic organisms‚ while phycobilins
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Spectrophotometer Lab: Length: 1437 words (4.1 double-spaced pages) Rating: Red (FREE) - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Spectrophotometer Lab Introduction: A spectrophotometer is an instrument that measures the quantitative amounts of light of different wavelengths absorbed and transmitted by a pigment solution (Mitchell‚ Reece). The spectrophotometer includes a light bulb‚ a reflector‚ and a detector. When a sample is in place and the chamber lid
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Lab Report 4 Biology 103 Prof. Dr. Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of
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