Abstract: During the lab a better agent for absorbing Procion Red Dye was trying to be found. Using the different dilutions of a stock solution and a spectrophotometer an Absorbance vs. Concentration graph was created and using the trendline from that graph it was determined which agent‚ zeolite‚ magnetic zeolite‚ or charcoal were better at absorbing the dye. From the results collected it was found that charcoal was the best at absorbing the dye‚ when moles of dye per gram of agent used were calculated
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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solution is directly proportional to the absorbance. The extinction coefficient relates the absorbance to concentration. It is specific for each individual compound and can be determined from the standard calibration curve by solving for ε‚ as I know the absorbance and concentration and can measure the width of the cuvette. Abs = ε bc In this equation‚ the absorbance is equal to the concentration of the CaCl2 (c)‚ multiply by the length of the path the light takes through the cuvette (b)‚ and then
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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According to the results‚ there was no significant difference between the filtered 100% water composition‚ and the filtered 90% water 10% soap composition. However‚ based on fFigure 2‚ the Average of Absorbance graph‚ showed that growth of the algae for the 100% water composition was observed to be slightly higher than the 90% water 10% soap composition. Meaning the soap may have slightly affected the growth of the algae‚ in some way. One possible‚ reason for a slightly higher growth for the filtered
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laboratory eight was to observe the affect of acid and alkaline cooking pH on pigments and textures of the vegetables(Yuan‚ 2014a) . Students were meant to gain experience on the different methods of processing fresh fruit and vegetables‚ both by observation and practise (Yuan‚ 2014a). The different methods were the effect of pH on pigment and texture of various vegetables‚ the effect that cooking procedures has on various pigments and flavours‚ the effect of sugar on texture and flavour of cooked fruit
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Tittle: Separation of leaves pigment. Introduction The photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis
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1. INTRODUCTION SilCote aluminum pigment is manufactured by mixing aluminum paste with Monomer‚ SR-350 (trimethylolpropane trimethancrylate) and additive‚ Z-6040 which is an epoxy-functional Silane (3-glycidoxypropyl trimethoxy silane)‚ in a jacketed mixer with the presence of catalyst‚ ABIN (azo-bis-isobutyronitrile). White spirit‚ acts as the carrier solvent in the process. 2. PROCESS FLOW The aluminum paste is first loaded into the jacketed mixer‚ followed by introducing a pre-determined
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different conditions to determine if the equilibrium constant is really constant. Fe3+ (aq) + SCN– (aq) ←→ FeSCN2+ (aq) A big part of this lab includes understanding (and in the end proving) that absorbance is directly proportional to the concentration of a solution (Beer’s Law)‚ and if Absorbance v. Concentration is graphed‚ a straight line will result. The regression line’s equation is used to determine the concentration of an unknown solution once the percent of T has been measured. The equation
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this lab was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment in chloroplasts. The chemical
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