Chromatography and a Spectrophotometer In this experiment‚ the separation of particular pigments‚ contained within a fresh spinach leaf‚ were examined. Paper chromatography is the process of separating certain molecules‚ or pigment molecules‚ based upon their polarity. Four different pigments were examined from the spinach leaf: chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and carotene. Based upon looking at the pigments individual molecule structures‚ our group hypothesized that the most polar molecule
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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oleracea and Musa acuminata‚ which use different wavelengths of light to display various pigments of color. The light energy for photosynthesis calls for an array of light to be absorbed by the plants. In this experiment‚ the different wavelengths of light can be traced and graphed by extracting chlorophyll from these two plants and placing them into a spectrophotometer‚ which identifies what wavelengths of light a pigment absorbs. It was found that Musa acuminata‚ commonly referred to as banana palm‚ showed
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Vitiligo Vitiligo is a skin condition‚ and sometimes is referred to as the Michael Jackson Disease. It is a skin condition in which there is a loss of pigment from areas of skin. That then results in irregular white patches of skin. Vitiligo occurs when the melanin forming cells‚ also called melanocytes fail to produce melanin. Melanin is the dark pigment that is in the epidermis that gives us our skin color. When the melanocytes fail to produce melanin‚ white patches of skin appear on the skin. Although
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Hypothesis Organic solvent and high temperature will destroy the cell membrane and make it permeable to the red pigment. The higher the concentration of the alcohol‚ the greater will be this effect. Principle Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes. Beetroot contains a red pigment called betalain‚ which is located in the large central vacuole of the beetroot cells. The vacuole is enclosed
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Question 1: You have carried out an investigation into the pigments that make up chlorophyll. Use the chromatogram you obtained when carrying out this investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary
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small amount of the unknown (approximately the tip of the spatula was used for all the pigments) was added into each clean dry test tube. For the first test 1 mL of 3 M of HNO3 was added. As soon as the acid came into with the solid‚ the pigment was observed to dissolve while also turning the solution into a pale clear colour. The same amount was used for the HCl reagent. As soon as the acid touched the pigment it yielded in a clear solution (solid dissolved after a few shakes). When the KI was added
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4.d.p 0.0426 0.0606 0.1667 0.1538 0.1250 0.1176 0.0976 It is evident from the graph and the table above‚ that as the temperature is increased‚ the amount of light passing through the tested solutions is decreased‚ and therefore the amount of pigment released from the beetroot samples is increased. The results produce a graph with negative correlation‚ a curve that has a negative gradient. The graph shows that as the temperature is increased‚ the colorimeter reading is decreased. The structure
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HSC Chemistry First Hand Investigation 2009 Scientific Report Aim: To carry out a fist hand experiment in the extraction of red cabbage pigment‚ use this extracted pigment to standardize against know pH’s‚ test 5 household items to determine the possible pH of these items by using the standardization and assess the pigment’s ability to be used as a Universal Indicator. Risk Assessment: When dealing with fire‚ the Bunsen burner should always be switched to the safety flame before lighting.
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This experiment is concerned with identifying photosynthetic pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the
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