ABSTRACT Rising energy prices and environmental problems led to the increase in alcohol demands in many aspects‚ like in making fuels. Many people are doing researches about making their own product so as to lessen their everyday expenses. Right now‚ Filipino scientists are in think of the search for alternative fuels‚ with the experts from the University of the Philippines in both Diliman and Los Banos campuses searching all over the archipelago for energy sources from the forests to the bottom
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extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon adding a solvent mixture of hexane and acetone‚ the chlorophyll and carotene pigments can be
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which are it’s light-capturing pigments. Colors in the wavelengths are either absorbed or reflected by the chlorophyll in which case green is reflected and colors like red and blue are seemed to be absorbed. If blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
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Wavelengths of Light and Pigmentation Abstract The roles played by light and pigmentation in photosynthesis were examined in the geranium and Coleus plant. In addition‚ the separation of certain pigments based on their molecular structure was also examined‚ with the use of paper chromatography. When different leaves of the geranium plant which were exposed to different wavelengths of light‚ photosynthesis proved to be most actively present in those exposed to blue and red wavelengths of light
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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Separation and Identification of Plant Pigments by Paper Chromatography Biology 1107L Introduction All living organisms require energy for their chemical processes. The ultimate source of this energy is the sun. Plants convert light energy into the chemical energy of sugars. During photosynthesis pigments are used to capture light energy. Pigments of green plants can easily be separated and identified using a technique called paper chromatography. The purpose of this experiment
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Hypothesis. If pigment from euonymus alatus (burning bush) leaves is extracted‚ then carotenes and xanthophylls will appear when a solvent‚ made of 9 parts petroleum ether and 1 part acetone‚ goes up Whatman #1 filter paper Purpose. The purpose of this experiment was to demonstrate the presence of carotenes‚ xanthophylls‚ and chlorophylls in leaves. Methods and Materials. A piece of Whatman #1 filter paper was cut to fit into an ignition tube. One end was cut to form a V‚ and the sides of
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Plants contain many pigments in different proportions. For flowering plants it’s mostly a combination of chlorophyll-A‚ chlorophyll-B‚ pheophytin and Carotenes. These pigments have shades of green‚ blue green‚ grayish‚ and yellow-orange. Their concentration is different for different species and also depends on the time of year. Also‚ these pigments have different solubility in different solvents. Extracted pigments could be used in another project‚ such as chromatography. Attention! This
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The other aspect of the lab includes Paper chromatography‚ which is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at d
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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