SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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Gas chromatography (GC) is a chromatography technique where the separation of individual components (analytes) from a sample relies on their differing distribution between a mobile and stationary phase. The mobile phase carries the analytes through the stationary phase. In GC‚ it’s an inert gas (usually helium or nitrogen). The gas must be inert‚ so it won’t react with the sample to give a false reading. The stationary phase is a substance fixed in place to which the sample adsorbs because
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The Art of the Conclusion The conclusion of an essay should establish a sense of closure or completeness that leaves the reader with lingering thoughts about the topic. Each writer has their own approach in composing a conclusion that they feel solidifies their argument; every approach makes different demands of a reader because of the moves the writer chooses to make. The essays “How to Tame a Wild Tongue” by Gloria Anzaldua‚ “Translating Translation” by Alberto Alvaro Rios‚ and “Joyas Voladoras”
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could help people. This could also help with learning new things. Listening to their opinion of their argument can even change the earlier opinions someone had. Seeing from another person’s point of view can help people with thoughts‚ understanding‚ conclusion. Someone’s point of view can make people’s thoughts work more. Trying to see from someone’s view will make people think of different ways to view what the other person sees. The thoughts would be why‚ when and where. With so many thoughts someone
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Conclusion Worksheet Instructions: Copy and paste the following text into a document or create a document that contains the same information. Respond to the following with complete sentences. 1. Copy and paste the introduction to your essay in the space below. King’s famous “Letter from Birmingham Jail‚” was written in response to a public statement of concern and caution issued by eight white religious leaders of the south. Even though King felt that they were men of “genuine good will‚” he didn’t
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CHEM 3152-003 Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C
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Candy chromatography My experiment is called candy chromatography. This project is mainly about the colors that are mixed with other colors to make candy markers Koolaid and much more. I think that only the color of the candy is going to show up on the coffee paper. I think that threw the entire experiment the color in the candy is going to show up the entire time. I think this is going to happen because most of the colors are solid colors. The material and equipment used was a sample of candy
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Introductions and Conclusions Introductions and conclusions play a special role in the academic essay‚ and they frequently demand much of your attention as a writer. A good introduction should identify your topic‚ provide essential context‚ and indicate your particular focus in the essay. It also needs to engage your readers’ interest. A strong conclusion will provide a sense of closure to the essay while again placing your concepts in a somewhat wider context. It will also‚ in some instances
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well written. What kind of conclusion does the writer use? The conclusion was very well written. I think his intentions were to leave you wondering‚ because it left me wondering. What is the effect of that kind of conclusion on this essay? He knew exactly what and how he wanted to write. The simple question from the beginning just attracts your attention. Very well written and powerful essay‚ this is just a very touching essay he wrote this from experience. Conclusion Essay What article did
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Background Information and Research Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored‚ especially pigments. This can also be used in secondary or primary colors in ink experiments. Is it used for Contaminants in rainwater‚ Analysis of narcotics‚ and Detection of substances in urine. Purpose • The purpose of this lab is to see the separation of dye on the candy. Data and Observations Marker Color
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