Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on
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In conclusion‚ after conducting the lab‚ the emergent properties of water were tested and proved. These trials and experiments were able to verify the adhesion‚ cohesion‚ polarity‚ temperature stabilization‚ solvency‚ and density changes exhibited by water. During the glass and wax paper lab‚ we observed the adhesive properties of water. When a droplet of water touched the glass‚ it dispersed immediately. The droplet placed on the wax paper remained intact and in droplet form. Also‚ when the
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Conclusion 1: In this first lab we attempted to discover which of the materials given to us were hydrates by heating them and then dissolving them in water. Our results showed that all of our substances were hydrates besides the sucrose‚ sucrose if the only one which did not both bring condensation when heated and dissolve in water‚ which agreed with our hypothesis because we also thought that sucrose was going to be the only one that wasn’t a hydrate. The only error that we may have encountered
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Lab Conclusion When comparing the average speed results from part two of the lab and the definition of acceleration‚ you find similarities between the two. First‚ average speed is distance divided by time‚ and we use it to describe the motion of an object moving at changing speeds. We can see this from our lab results from the average speed of the marble traveling down the ramp‚ because it picks up speed. When the marble is released at the top of the ramp‚ the ball doesn’t have the same momentum
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Conclusion In this experiment‚ we measured the mass of 4 gasses; oxygen‚ carbon dioxide‚ helium‚ and lab gas. We took a Ziplock bag‚ and turned into a non-stretchy balloon‚ and filled with each gas and measured the apparent mass then calculated the actual mass‚ then find a ratio between the mass of oxygen and the other gasses. We tried to keep the pressure about the same each time so our calculations would be more accurate. We found out that the heaviest was carbon dioxide‚ and the lightest
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Conclusion to Motion Lab Kerreon Wright 3rd Period Ms. Gislason The purpose of this Motion Lab was to find the acceleration of a steel marble going down a straight track six different times to figure out how an object’s mass affects acceleration. It doesn’t due to Newton’s second law of motion. There were six different accelerations for each trial and they are: 7.88 m/s squared‚ 6.78 m/s squared‚ 6.07 m/s squared‚ 5.57 m/s squared‚ 4.32 m/s squared‚ and 5.11 m/s squared. It’s possible
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In this Laboratory experiment‚ my lab partner Alexander and I were able to understand thoroughly the physical and chemical properties of salt (NaCl) and sand (SiO2). Followed by the right procedure we were able to design and test out the components of both NaCl and SiO2 in order to separate the unknown mixture that we were able to find out about. We were also given the task to provide the percent composition of the mixtures. Therefore‚ the separation of components among this experiment allowed us
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Lab Conclusion on Density In the lab of measuring density‚ precision and accuracy are very key components. Precision is the exactness of a measurement or good technique. Now accuracy is the value that we measured compared to the true value that the book has. Precision and accuracy are important because you need to have good technique and have your value close to the true value to minimize the error analysis. If you do these steps correctly you are a good chemist but if you do them poorly they
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In conclusion to the distillation lab‚ I think our data shows that our experiment went well. Our graph resembles the graph in the lab handout (graphs attached to lab notebook sheets). The fractional distillation shows the sharp slope between when the cyclohexane burns off and mostly only toluene remains. The microscale graph seems to be accurate because it shows the large jump where the cyclohexane is burned off. To view the apparatuses used in the experiment look at the attached pictures. To
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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