Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
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ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________ _________________________________ Abstract: The experiment was done to separate and analyze the components of chili pepper. Chromatography was used because of its
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Introduction: Hypothesis: My hypothesis for this experiment‚ the study of the effects of different wavelengths on leaf pigments‚ if chlorophyll a absorbs blue-violet light‚ chlorophyll b absorbs blue light‚ carotene absorbs blue-green light‚ and xanthophyll absorbs blue light‚ then wavelengths of greatest absorption will be 400‚ 450‚ 500‚ and 450 respectively. Independent Variable: Wavelengths Dependent Variable: optimum wavelengths of light for absorption Standard Variable: method of
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on research and development have also become broader each day. Controversies have also emerged and one of the most discussed topics has been directed towards synthetic versus natural colorants. Food pigments are classified based on its origin or source; natural or synthetic. Synthetic food pigments are those substances that are needed to be tested for toxicity and allergenicity before
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Activity in Plants Using Different Wavelengths of Light using Chromatography and a Spectrophotometer In this experiment‚ the separation of particular pigments‚ contained within a fresh spinach leaf‚ were examined. Paper chromatography is the process of separating certain molecules‚ or pigment molecules‚ based upon their polarity. Four different pigments were examined from the spinach leaf: chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and carotene. Based upon looking at the pigments individual molecule
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Chromatography of Food Dye Chem 180-DL3 Abstract: The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance. In this experiment different dyes were used such as McCormick food coloring‚ M&M’s‚ Kool-Aid‚ and the dyes that came with the lab kit. Experiment and Observation: In this experiment a 50mL beaker was filled with luke warm water and a pinch of salt water was dissolved in it
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Introduction: Plant hormones and growth regulators are chemicals that affect flowering‚ aging‚ root growth‚ killing of leaves‚ promotion of stem elongation‚ color enhancement of fruit‚ prevention of leafing‚ and many other conditions. Very small concentrations of these substances produce major growth changes. All plants produce hormones naturally‚ and growth regulators can be applied by people to are applied to plants by people. Plant growth regulators may be synthetic compounds that mimic naturally
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Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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