It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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Analysis and Separation of Organic Acids in White Wine Using High Performance Liquid Chromatography Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Performed: December 9‚ 2011 Submitted: December 15‚ 2011 RESULTS AND DISCUSSION solutions containing increasing amounts of the organic acid. These solutions were contained in flasks numbered 5‚ 6‚ 7‚ 8‚ and 9. (Refer to
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CHEM 3152-003 Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C
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Plants‚ also called green plants (Viridiplantae in Latin)‚ are living organisms of the kingdom Plantae including such multicellular groups as flowering plants‚ conifers‚ ferns and mosses‚ as well as‚ depending on definition‚ the green algae‚ but not red or brown seaweeds like kelp‚ nor fungi or bacteria. Green plants have cell walls with cellulose and characteristically obtain most of their energy from sunlight via photosynthesis using chlorophyll contained in chloroplasts‚ which gives them their
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Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
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Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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Background Information and Research Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored‚ especially pigments. This can also be used in secondary or primary colors in ink experiments. Is it used for Contaminants in rainwater‚ Analysis of narcotics‚ and Detection of substances in urine. Purpose • The purpose of this lab is to see the separation of dye on the candy. Data and Observations Marker Color
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Discussion Using the Thin Layer Chromatography experiment‚ caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Caffeine contains four amine groups that are extremely polar as a result of the hydrogen bond and amide functional group. Acetaminophen was found to be the second most polar analgesic drug tested. Acetaminophen contains a polar alcohol group on one side and amide group on the other but also includes non-polar functional groups that consisted
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Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*‚ Phoebe Abalos‚ Kevin Antiga‚ Carmelus Aseneta 3-Biology 2 College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The group used five different analgesics in this experiment: aspirin‚ acetaminophen‚ ibuprofen‚ caffeine‚ and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate‚ it was placed under ultraviolet light for the spots resulted
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CHAPTER 4 RESULTS AND DISCUSSION 4.1 Identification of camphor and menthol using gas chromatography – mass spectrometry (GC – MS) In this study‚ the presence of menthol and camphor were determined from 5 different locally produced medicinal topical ointment samples. Sample of Vick® VapoRub® and Hurix’s Massage Balm were purchased from local pharmacies‚ Bunga Cengkih Balm and Serai Balm samples were purchased from the grocery shop. Buah Pala Balm sample was purchased from the night market
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