In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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Lab 4: Plant Transpiration Project By Shelby Hyde Lab 030 Date Due: March 12‚ 2013 The Effect of Wind on the Rate of Transpiration Introduction: Transpiration is the process through which water is evaporated from plants. This serves many purposes‚ including thermoregulation and the diffusion of CO2‚ but most importantly creates a water potential difference which causes the mass flow of water and nutrients from the roots to the leaves of the plant. Transpiration is accomplished through structures
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laboratory eight was to observe the affect of acid and alkaline cooking pH on pigments and textures of the vegetables(Yuan‚ 2014a) . Students were meant to gain experience on the different methods of processing fresh fruit and vegetables‚ both by observation and practise (Yuan‚ 2014a). The different methods were the effect of pH on pigment and texture of various vegetables‚ the effect that cooking procedures has on various pigments and flavours‚ the effect of sugar on texture and flavour of cooked fruit
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Fast Plants Lab Background Gregor Mendel is an Australian monk‚ who worked in a garden at a monastery. He experimented with pea plants‚ and soon became known as the father of genetics. The reason he studied pea plants was because they grow quickly‚ the traits can be easily observed‚ and the plant is easy to pollinate. Mendel’s method was to control the pollination of the pea plants and create offspring’s of the two plants that were pollinated together. Using self-pollination and cross pollination
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5.05 Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge‚ alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and
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The Wonders of Plants Experiment 2 – Upside Down Mungbeans Experiment 3 – Plant Labyrinth 6/18/2015 Mt Gravatt State High School Trinity Wong Abstract Introduction: Plants can’t move like animals do but they respond to certain stimuli‚ making them change the direction in which they grow. Plants are very sensitive to their environment and have evolved many forms of "tropisms" in order to ensure their survival. A tropism is a growth movement whose direction is determined by the direction
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favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This
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5.05 Ink Chromatography Basic Lab Components Background Information and Research: 1. Paper chromatography type of method that is used to separate mixtures of substances from a solution. 2. There are many uses for paper chromatography‚ especially concerning the fields of chemistry and biology. One use is to allow scientists to detect any sort of contaminants is rainwater. Another use is to identify clues at a crime scene such as blood‚ ink‚ or drugs. However‚ the more common use is to
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Gas Chromatography Purpose: The purpose of the gas chromatography lab is to find out how different substances interact with the surface of a solid. Chromatography is a separation technique that depends on the relative distribution of the components of a mixture between a mobile phase and a solid stationary phase. Chromatography measures the tendency of a substance to interact with the surface of a solid or to remain in a mobile phase. When doing a chromatography lab the mobile phase has to
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