Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine- used in the activity in application * glass slide- used in the activity in application(wet mount) * cover glass- used in the activity in application(wet mount) * ball of cloth- used in the activity in motivation * manila paper- used in the activity in lesson proper(table) * improvised canal- used in the activity in motivation * mud- used in
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What is the maximum magnification you will need? Are you looking at a stained specimen? How much contrast/resolution do you require? Next‚ start setting up for viewing. Mount the specimen on the stage The cover slip must be up if there is one. High magnification objective lenses can’t focus through a thick glass slide; they must be brought close to the specimen‚ which is why coverslips are so thin. The stage may be equipped
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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Compound Light Microscope Purpose: The purpose of this lab is to 1. Learn the parts of a compound light microscope. 2. The functions of those parts. 3. Proper use and care of the microscope. 4. Learn the technique of preparing wet-mount slides. Materials: * Compound light microscope * Soft cloth * Microscope slide * Cover slip * Dropper * Scissors * Newspaper Procedures: Part A. Care of the Compound Light Microscope. Step 1: Always carry the microscope
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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Methods: Simple Stain For this experiment‚ I used E. coli to make a heat fix smear. I placed a drop of water in middle of the slide‚ then put in small amount of E. coli and mixed well. The slide was allowed to air dry. When it was completely dry‚ I passed the slide over the flame for 3 times to fix the cells to the slide. For the staining part‚ I put a drop of methylene blue onto the smear for 30 second‚ rinsed with water‚ blotted dry and observed the slide using oil immersion.
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High Risk Pregnancy -pregnant with a concurrent medical disease -with pregnancy related complication -presence of external factor that jeopardizes the health of the mother‚ the fetus‚ or both [pic] Candidiasis (Moniliasis) INCIDENCE: • 75% of women will have at least 1 episode of VVC with 40-45% having 2 or more episodes ETIOLOGIC AGENT: • Candida albicans‚ Candida glabrata MODE OF TRANSMISSION normal flora of the skin & vagina not considered
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1 Biology 2070H - Lab #1 MICROSCOPY AND CHARACTERIZATION OF CELLS Introduction: Cells can be very difficult entities to study. They are usually very small and transparent‚ yet extremely complex. Fortunately‚ there are many tools available to the cell biologist that aid in their study. The light microscope is to this day perhaps the single most important instrument used in Cell Biology. It is used under bright field conditions to study the organization of cells in fixed and stained sections
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reported in table and drawings of my findings. Table 1 Specimens | “e” slide | Penicillium with conidia | Bacteria‚ bacillus form | yeast | Bacteria‚ coccus form | Bacteria‚ spirillium form | Prepared yogurt slide | Fresh blood smear | Fresh yogurt slide – 24 hours old | Observations Specimen | Magnification | Observations | “e” | 150x | “e” is clear to see | | | Edges are not crisp as rest of the image | Penicillium with conidia | 150x | Long‚ wavy‚ skinny strands
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mycobacterium. Procedure: Bacterial Smear Preparation 1. Prepare 2 Bacterial Smears of each broth culture of bacteria labelled A and B Gram’s Staining 1. Flood the two bacterial smears with crystal violet (primary dye/stain) for 10 seconds. 2. Wash the smears with running water. 3. Flood with gram’s iodine (mordant) and wait for 10 seconds‚ then wash with running water 4. Decolorize the smear with 95% ethanol until the thinnest parts of smear are colorless then wash with running
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