STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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Female Genitalia Health Assessment Lecture Objectives: * Describe the structure and function of the female genitalia * Describe particular developmental and transcultural considerations. * Collective subjective and objective data. * Record the findings. * Identify the appropriate nursing diagnoses for problem areas identified. Female Genitalia Structure & Function * Consists of external and internal structures External: * vulva or pudendum (extends from
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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infection Case 2: Normal blood smear Case 3: Normal blood smear Journal Questions for lab In what ways do normal red and white blood cells differ? White blood cells have a nucleus‚ red blood cells do not‚ white blood cells protect while red blood cells transport‚ red blood cells have a longer life span than white blood cells. Which type of white blood cell would you expect to be most common in a normal blood smear? In a normal blood smear‚ one would expect to find more neutrophil
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of the slide with a 45 degree angle. Remove some of the excess stain that’s on the slide using a tissue paper. To finish‚ view your slide on the COMPLEX STAINING microscope. Clean and dry microscope slides thoroughly. Flame the surface in which the smear is to be spread. COMPLEX STAINING 3. 4. 5. 6. 7. 8. Flame the inoculating loop. Transfer a loop full of tap water to the flamed slide surface. Reflame the loop making sure the entire length of the wire that will enter the tube has been heated to
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Methods: Simple Stain For this experiment‚ I used E. coli to make a heat fix smear. I placed a drop of water in middle of the slide‚ then put in small amount of E. coli and mixed well. The slide was allowed to air dry. When it was completely dry‚ I passed the slide over the flame for 3 times to fix the cells to the slide. For the staining part‚ I put a drop of methylene blue onto the smear for 30 second‚ rinsed with water‚ blotted dry and observed the slide using oil immersion.
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1320 HBL for CP2078 (Sample Preparation for Microscopy) Name of students Admission No Class Total Marks / 45 Assignment 1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink
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respectively. Only 19.2% had undergone a clinical braest examination within one year and 3.6% had ever undergone a mamography. Only 76.3% knew that a Pap smear detects precancerous stage of cervical cancer. Among 169 married workers‚ 73.4% had never had a Pap smear and only 17.2% had got it done within the preceding 5 years. Among the reasons for not doing a pap smear within 5 years‚ 47.0% belived it as not nescessary‚ 17.3% due to fear/dislike‚ 23.2% as not having symptoms‚ 3% had not known about it and 3%
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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