GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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Methods Smear Preparation Obtained a clean dry glass slide‚ Staphylococcus aureus and Pseudomonas cultures. Added drop of water onto the center of slide. 3. Flame-sterilized inoculating loop‚ top of culture tube‚ obtained a very small sample of bacteria and smeared onto the water drop on the slide slide Repeated smear with second bacteria culture tube. Smear was allowed to air dry and was subsequently heat-fixed onto the slide. Gram Stain The S. aureus and P. fluorensens smear was covered
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CHAPTER ONE INTRODUCTION The industrial attachment is an important part of the course structure of the university. This is the time students appreciate the theoretical work done in the lecture halls when seen practically. This attachment introduces the student to the outside world in terms of working experience and also prepares them for the future. The department of applied biology therefore introduced the industrial attachment program in its learning curriculum to give students a chance to combine
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from the slant S. aureus into the drop of water. Mix both cultures on the slide with an inoculating loop to make a mixed smear. Air dry and heat fix this smear. Cut a sheet of bibulous paper to fit the dimension of the slide and place this paper on top of smear. When water is boiling‚ place the paper-covered slide on top of the beaker. Add carbolfuchin to the paper covering the smear. Steam the preparation for 10-12 minutes. Do not let the paper dry out. Add more carbolfuchin to keep the paper wet while
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Question 1 | | 1 / 1 point | Microbes are not responsible for ________. | | synthesis of acetone and alcohol | | | breaking down wastes | | | synthesis of vitamins | | | antibody production | | | oxygen generation via photosynthesis | View Feedback | | Correct! | Question 2 | | 1 / 1 point | The cell theory states that ________ are made up of cells. | | protozoa | | | archaea | | | plants | | | all living things | | | animals | Question
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*Cancer* Cancer (medical term: malignant neoplasm) is a class of diseases in which a group of cells display ’’uncontrolled growth’’ (division beyond the normal limits)‚ ’’invasion’’ (intrusion on and destruction of adjacent tissues)‚ and sometimes ’’metastasis’’ (spread to other locations in the body via lymph or blood). There are over 150 types of cancers. Cancers in teens:- 1. Osteosarcoma (Pronounced: os-tee-oh-sar-koh-muh) is the most common type of bone cancer.
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Ms. Jenette Malaban Activity 6: Fecal Analysis for the Detection of Parasites I. Objectives: 1. T o perform the basic techniques in fecal and blood analyses. 2. To identify parasitic forms that are recognizable in the fecal samples and blood smears by microscopy. 3. To determine the advantages and limitations in each procedure. II. Methodology Before the activity of checking or detecting the presence of parasites to the stools‚ it was started with getting a stool samples from the grade
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stain pink with the Gram stain. METHOD: Obtain a glass slide‚ placing a tiny drop of water to the slide. Bacteria is then gathered from a previous streak plate‚ then smeared in a circular motion on the glass slide along with the water. Allowing the smear to air dry. Once air dried‚ the glass slide must be heat fixed (fixing the bacteria to slide to prevent washing away later during staining)‚
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Unit 10: Blood/Immunology Case Study Lab What were your three diagnoses? 1. Case 1: normal blood smear 2. Case 2: acute lymphocytic anemia 3. Case 3: mononucleosis Journal Questions for lab 1. In what ways do normal red and white blood cells differ? Red blood cells are easier to see under high levels of a microscope and white blood cells are better seen under low levels. Also white blood cells are used to fight off infections as for red blood cells carry oxygen
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Beneficence in Medicine I. In my judgment‚ the physician and care team should give general anesthesia to Ms. R. and perform the Pap smear. II. The ethical principle that best supports my position is beneficence. III. Beneficence can be defined as acts of mercy and charity‚ but can be expanded to include any act that benefits another (Edge & Groves‚ 1999). The three statements of beneficence as stated by Edge & Groves (1999) are: 1. One ought to prevent evil or harm. 2. One ought to remove
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