Wintersemester / page 1 Experiment 22 Isolation of plasmid-DNA from bacteria and PCR Advisor Konrad Egli: kegli@botinst.unizh.ch Reading Chapters in BBOM 10th: 10.8 BBOM : Madigan M.T.‚ J.M. Martinko and J. Parker: "Brock - Biology of Microorganisms"‚ 10th Edition (2003)‚ Prentice Hall. Objectives Background • Isolation of plasmid-DNA from different bacteria clones • Handling of bacteria clones • PCR-experiment The typical plasmid is a circular double-standed DNA molecule
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2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
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Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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Restriction Fragment Length Polymorphism and Southern Blotting 1. Abstract The aim of the experiment was to be introduced to the techniques involved in the identification of restriction fragment length polymorphisms. Restrictions were carried out using three different restriction enzymes‚ ECORI‚ HindIII and BstELI with their buffers. Lambda (λ) DNA was then examined using electrophoresis and Southern blotting. The results showed that λ DNA was best digested by EcoRI as all of the expected bands
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Isolation‚ Morphological‚ and Biochemical Characterization. A high-throughput screening of cartenoid-producing microorganisms resulted in 25 astaxanthin-producing bacterial strains from marine water samples that were collected from the Pacific coast of Japan (unpublished results). Among the isolates‚ a novel‚ highly selective astaxanthin-producing marine bacterium‚ labeled strain (N-5)‚ was isolated based on its ability to produce orange pigment on NA plate after 2 days of cultivation at 30 °C. Strain
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uses of plasmids in G.M. experimentation. Plasmids are extrachromosomal genetic elements found in a variety of bacterial species. They are double stranded; autonomously replicating‚ supercoiled‚ covalently closed circular (ccc) DNA molecules that range in size from 1 kb to greater than 200 kb. Often‚ plasmids contain genes coding for enzymes that‚ under certain circumstances‚ are advantageous to the bacterial host (Table 1). Table 1. Some of the phenotypes conferred by different plasmids that
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Chemistry Lab Report 1 Nicole H. Healey (Experiment 1 and 2) October 7‚ 2014 Data Collection: Table 1: (First Titration) C2O42- Analysis Sample 1 Sample 2 Molarity of KMnO4 0.02m 0.02m Weight of Sample 0.237g 0.225g Final Buret Reading 28.5ml 26.3ml Initial Buret Reading 0ml 0ml Volume of KMnO4 dispensed 28.5ml 26.3ml Moles
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Leah Romero 04/18/2018 Lab 9 Conclusion Chem. 102L For lab 9‚ isolation of casein‚ the main purpose was to take the milk sample that was provided in the lab and through the addition of acid‚ be able to find the pH of the milk at the isoelectric point of casein. Isoelectric point is the pH at which a particular molecule carries no net electrical charge. The main points of the lab or the outcomes we were looking for were to be able to look at the properties of proteins in casein and be able to identify
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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Abstract: This experiment synthesized H-Gly-Phe-OH dipeptide using “Fmoc chemistry”. The first part of experiment was the synthesis of L-phenylalanine methyl ester hydrochloride. The methyl ester can be synthesised by reaction of thionyl chloride‚ SOCl2 and dry methanol with L-phenylalanine under reflux condition. The peptide bond was formed later in the experiment‚ where HBTU‚ DiPEA and a solution of Fmoc-Gly-OH in DMF were added to a solution of L-phenylalanine methyl ester hydrochloride in DMF
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