2014. Title: Ligation of Lambda DNA pre-digested with EcoRI and HinDIII. Restriction of Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme
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Mr. Mihordea Motion of Atoms and Molecules lab summary In this lab the standard heat of combustion of magnesium was calculated by using the calorimeter. Also‚ the point of combustion of magnesium was to see how much heat would be released from fireworks such as sparklers. The concept of this lab was to find the difference in temperature from the beginning to end of combustion of magnesium and calculate the standard heat
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Transpiration Formal Lab Report By Jessica Tran Introduction When water is transported from the roots to the mesophyll cells in the leaves‚ it is evaporates out the stomates‚ called transpiration‚ to create a lower osmotic potential. Osmotic potential is the part of the water potential of a tissue that results from the presence of solute particles. Even though the stomates open to release water‚ it also brings in carbon dioxide to produce sugar and oxygen through a process of photosynthesis
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Objective: The objective of this lab is to determine and analyze the many chemical qualities of copper ‚ by using many solutions that enable copper to undergo chemical changes that will take copper from a metal back to its original state. This is done using cooper‚ Concentrated HNO3‚ 6 M NaOH ‚ Bunsen burner‚ 6 M H2SO4‚ Mg ribbon‚ and two test tubes. The techniques used in this lab which is cleaning glassware‚ disposing of chemicals ‚ measuring mass ‚ centrifugation ‚ venting gases‚ and test tube
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In this lab‚ there was a comparison between accuracy and precision of densities measured by volume. Mass measurements are often used units in the metric system. Volume can be examined a borrowed unit. The volume measurements were made in mL. Every measurement had a level of hesitancy affiliated with it. The experiment could feel uncertain from the object used for measurement and from the accuracy of the person doing the measuring of the volume. Because of this uncertainty‚ the experiment must be
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Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
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the terrorized Chicago in the late 1800’s‚ yet he was a slave to his own mind and his own disorders. Herman‚ who is also famous as H. H. Holmes had two personality disorders that drove him to commit the actions that he committed. evidence shows that Holmes had personality disorders like narcissistic personality disorder and antisocial personality disorder. H. H Holmes shows signs of antisocial personality disorder in many ways. To begin with‚ Mudgett started at an early age torturing animals
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ab reportChemistry 117L Laboratory Report Name: Aneesa Noorani Lab Day: Tuesday Lab Room: SCL 114 Date of Experiment: January 22‚ 2013 TA: Mikhail 1. Basic Laboratory Skills Purpose(s) of the Experiment: The purpose of the first part of today’s experiment is to establish the stoichiometry of the reaction between titrate oxalate (C2O42-) and permanganate (MnO4-). The purpose of the second part of today’s experiment is to learn about the concepts of the rate of chemical reactions
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The purpose of the lab was to measure the effectiveness of antacids at neutralizing excess stomach acid. A neutralization reaction is when an acid and a base react to form water and salt. To illustrate‚ H+ hydrogen ions from the acid react with the hydroxide ions OH- from the base. Antacids are taken to relieve heartburn or indigestion caused by excess stomach acid. For instance‚ antacids such as alka-seltzers contain baking soda which is highly reactive with acids‚ settling the discomfort of indigestion
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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