I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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Materials and Methods Growth condition of clinical isolates and determination of MIC: Twenty clinical isolates of H. pylori were obtained from patients suffering gastritis and duodenal ulcer who referred to Al- Zahra Hospital‚ Isfahan for gastrointestinal endoscopy in a year of 2011. The gastric biopsy specimens were homogenized and cultured on Brucella Blood Agar (Merck‚ Germany). Skirrow’s supplement including polymyxin B‚ vancomycin and trimethoprim (Merck‚ Germany)‚ 5% defibrinated Sheep’s Blood
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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H&M In Australia Analysis Report Course Leader: Cai Huan BU1401- Business Environment Class: IB 3 Date: 14th June 2013 Group members: Joe Johnny Jacky Jesse Scott Executive summary This report main analysis feasibility of H&M enter Australia. The first section review the H&M company and products by their design style. The second section review
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Report by Analysts of Group 6. Investment Recommendation:HOLD (Current stock is fairly priced) Business Analysis Overview The Swedish company Hennes & Mauritz AB is the second largest clothing retailer in the world‚ just behind Spain-based Inditex (parent company of ZARA). The H&M Group’s business consists mainly of sales of clothing‚ accessories‚ footwear‚ cosmetics and home textiles to consumers. In addition
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Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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HISTORY & PHYSICAL EXAMINATION REPORT Patient Name: T. J. Moreno Patient ID: 110497 DOB: 02/15/- - - - AGE: 44 SEX: M Room No.: 502 Date of Admission: 05/10/- - - - Admitting Physician: Patrick Keathley‚ MD‚ Endocrinology Chief Complaint: Left ankle pain. DETAILS OF PRESENT ILLNESS: This is a 44-year-old Hispanic male‚ when was kindly asked to admit by Dr. Max Hirsch the patient is status post arthrodesis of the left ankle and has newly diagnosed diabetes
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Table of contents 1. Introduction 2. Macro environmental analysis (PESTLE- political‚ economic‚ social‚ technology‚ legal‚ ecological) 3. Customer analysis 4. Market description 5. SWOT analysis (Strengths‚ weaknesses‚ opportunity‚ threats) 6. Conclusion and Recommendations 7. References P.1 D002
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Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the
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Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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