The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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Photosynthesis Lab Report Purpose: To research the effect of different wavelengths (colors) of light on plant growth during photosynthesis. Background Information: Photosynthesis is the process by which sunlight is captured by chloroplasts within plant cells and turned into energy. This energy is used to help the plant grow roots‚ leaves‚ flowers and fruit. Energy is required by living things
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Biomolecular Sciences‚ 4th Ed.‚ 2013‚ Longman Willis‚ J. (2004) Data Analysis and Presentation Skills: An Introduction for the Life and Medical Sciences. Wiley‚ Chichester. APPENDIX- Safety Assessment 1. General Aspects Gloves must be worn at all times to protect from pigment staining. Safety glasses must also be worn throughout the lab to protect the eyes from chemicals. Potential injury may arise from razor blades and cork borers. Lab coats must be fully fastened to protect clothes from staining. 2
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H&M Analysis Summary H&M is a Swedish company founded in 1947. It is a family owned company controlled by Stefan Persson and family. H&M act in the fashion industry and are one of the really big global players with 2078 stores in more than 30 countries. In 2009 they launched H&M Home which sells interior textiles through internet. Recently H&M have launched several new store concepts. Other than the H&M stores there are now also Weekday‚ Monki‚ COS and Cheap Monday stores. H&M’s new concept stores
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turn off the primary production. Darkness has no effect on respiration. This is because cellular respiration is actually the reverse process of photosynthesis. Oxygen is a necessity of life requirements for basically all living organisms.* In this lab we are testing how different levels of salinity in the water indirectly affects the gross primary productivity in aquatic plants. To measure this you would use the light and dark bottle method. Only respiration (R) can occur in the bottle stored in
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agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas species. If the Pseudomonas species
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Biology‚ Bio 110; October 1‚ 2014 Observing Membrane Structure and Observing Effects of Chemical stress on Membrane Crystal Eve Lopez‚ Dr. Barua Madhabi Keywords: beet root model system‚ spectrophotometer‚ betacyanin‚ cellular membrane‚ phospholipid Abstract The cellular membrane separates and protects the cell acting almost as a wall. Depending on what stressors there are the cellular membrane can become damaged. The objective of this experiment was to examine the struc
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franciscana are known to live in high salinity lakes that are often basic (Biology 108 Lab Manual 2015). Furthermore‚ A. franciscana feed on photosynthetic phytoplankton which inhabit areas of light availability but are also more susceptible to predation in highly-lit areas (Biology 108 Lab Manual 2015). Also‚ A. franciscana can withstand a broad range of temperature except extreme values may affect survival (Biology 108 Lab Manual 2015). In this experiment‚ the habitat
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Organismic Lab Lab1- Dichotomous Key Introduction A dichotomous key is a series of paired statements or questions that are used to categorize organisms with their similarities in characteristics and or structure. The word dichotomous comes from two Greek words that translate to “divided in two parts.” In a Dichotomous key each step has two choices: whether a particular characteristic is present or absent. The questions
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