1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
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The Expression of Absurdism In Albert Camus’ Novel “The Stranger” The Stranger by Albert Camus is a novel written in an absurdist point of view. The main character Meursault is faced with the death of his mother‚ the conflict between his neighbor Raymond‚ and Raymond’s girlfriend‚ and finally the decision to shoot the Arab. Camus suggests through his main character Meursault that being an absurdist is not living a life without meaning but rather living a life with a meaning while admitting
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A History on Universal Facial Expressions: The Works of Lamarck‚ Izard and Russell Kathleen Coyne-Boyles From the time of the ancient Greeks through to the modern age‚ understanding and interpreting man’s emotions and body language have been a source of both fasicnation and a point of intellectual debate. The ancient philosophers‚ such as Aristotle‚ proposed the idea that the face takes on varying appearances which are characteristic of each of the passions (or emotions) of humankind. The famous
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In mathematics‚ an algebraic expression is an expression built up from constants‚ variables‚ and a finite number of algebraic operations (addition‚ subtraction‚ multiplication‚ division and exponentiation by an exponent that is a rational number).[1] For example‚ is an algebraic expression. Since taking the square root is the same as raising to the power ‚ is also an algebraic expression. A rational expression is an expression that may be rewritten to a rational fraction by using the properties
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Facial expressions comprise a considerable amount of nonverbal communication. With our 80 facial muscles we can create more than 7‚000 facial expressions. It is one of the most difficult types of nonverbal communication to master the subtle meanings and to be able to catch micro-expressions. Some facial expressions are similar where ever we are in the world. Human faces communicate happiness‚ sadness‚ anger and fear. The overall appearance of the face offer information about age‚ sex‚ race‚ ethnicity
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Running Head : SIMPLIFYING ALGEBRAIC EXPRESSIONS 2 Simplifying Expressions Be sure to have a centered title on page 1 of your papers . [ The introductory paragraph must be written by each individual student a nd the content will vary depending on what the student decides to focus on in the general information of the topic. YOUR INTRODUCTION SHOULD CONNECT MATH CONCEPTS AND REAL - WORLD APPLICATIONS. DO NOT INCLUDE THE DIRECTIONS IN THE INTRO! The following paragr aph is not an
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Freedom of Expression There are some issues and problems that are very hard to solve. Freedom of expression is one of them. Its hardship springs from the ambiguity of the term freedom itself. Philosophers always ponder on the question as to whether freedom has its own bounds‚ or it referres to the absence of any kind of restrictions. There are some people asserting that people can express themselves however they want‚ and all kinds of expression should be tolerated. However‚ In my opinion
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ways. Artists use images‚ songs and words to explain how they see the world. Furthermore‚ art expressions could be against the government ideas‚ consequently artists could be restricted on show their thoughts. Firstly‚ obscene artist expressions in videos and pictures should be restricted by the government because it has to keep the order and the good customs in its country. Secondly‚ some art expressions like graffiti make the streets very ugly and dirty so‚ country’s authorities should regulate
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Two: Art as Expression‚ Or Just Aesthetics The question of what art is cannot be properly answered without asking why art is. Prior to the advent of the written language‚ art was used as a means of communication‚ and in some ways‚ written language is in its own regard‚ art. Art‚ then‚ must be an expression of meaning by the artist‚ or potentially by the client that artist created the artwork for‚ but this assumption is altogether too broad. Art is not exclusively a private expression because it
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