transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism. A gene is a piece of DNA that
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MANAGEMENT PROJECT REPORT Letter of Transmittal: Sir Jawad Bhatti‚ Subject: Operations Management Report on Lux Soap Respected Mr. Jawad Bhatti‚ We present to you our Report on the operation management of Lux‚ a part of our term report. The preparation of this term report was a challenging task but it provided us with the much-important knowledge about the operations of Lux soap. We have put in great efforts in the preparation of this report and have tried hard enough to match the requirements
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Identification of Mystery Plasmids Abstract Plasmids serve as important tools in genetics and biotechnology labs‚ where they are commonly used make many copies of a particular gene. The aim of this experiment was to identify three mystery plasmids based upon their characteristics; such as size‚ antibiotic resistance‚ lacZ profile and conjugative properties. The results obtained showed that plasmid number2 was the pDSK519 plasmid and its size was 26229.58 Bp. Plasmid pDSK519 also was found to be
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The Changes of Expression In this paper i will discuss the way expression has changed from the way it was in prehistoric times to the present time. The three items that have evolved are non verbal communication‚ speech‚ and the writting format. First of all non verbal comminunication the has evolved by simple movements to sign language. what is non verbal communication? The sending and receiving of messages thru movements of the body‚ postures and expressions of the face. Example the shking
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Introduction Plasmids are circular‚ double stranded extrachromosomal DNA molecules that are found in bacteria which can self-replicate. They are naturally occurring DNA molecules advantageous to the host bacterium by carrying genes which specify metabolic capacities. (Garrett et al.‚ 2010) Besides‚ plasmids exist in a wide variety of sizes from a few thousands to hundreds of thousands of base pairs. Many plasmids have been engineered to serve as plasmids cloning vectors to carry genes. (Synder et
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Product Profile History of LUX Industry Analysis Swot Analysis Segmentation Basis Marketing Mix Product Life cycle EXECUTIVE SUMMARY Lux Soap came into existence in 1964. Lux occupies a large market share in the Pakistani soap market as well as the international arena. The new Lux‚ with the sunscreen formula‚ is targeted towards a different market segment as compared previously to previous Lux. Lux is the first brand to bring the sunscreen concept in soap industry. Lux plans to cater to all these
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The Swimming Pools by Thomas Lux is a poem that talks about the rich and the poor. Lux use 5 kids at an apartment complex pool‚ one fat kid‚ one insecure girl‚ and three other kids to represent the different social and economic classes we have in society today. Lux compares these kids to the poor‚ the lower middle class‚ and the rich. He uses the innocence of the kids at the pool to get the idea that the rich always are cruel to the poor‚ and he uses the insecure girl to show the sympathy the lower
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to enjoy that moment when anything seems possible. And that’s just what Lux offers you on a daily basis at a price you can afford. Glamour factor Lux introduces a luxurious new range of body and hand washes to indulge the senses with promises of renewal and relaxation. Making your day just that extra bit special. The rich‚ creamy lather will indulge your body and leave your skin feeling pampered and deliciously silky. LUX has always been synonymous with beauty and the enigma of a woman. Since
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Contents and supplementary information for: Principles of Gene Manipulation Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Gene manipulation: an all-embracing technique Basic techniques - (POGC02.pdf‚ 1‚560KB) Cutting and joining DNA molecules Basic biology of plasmid and phage vectors Cosmids‚ phasmids and other advanced vectors Cloning strategies Additional updated information on Cloning strategies Sequencing and mutagenesis Cloning in bacteria
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protein into pET41a(+) Plasmid Introduction The overall purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli)‚ a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way‚ from cloning and using recombi- nant research‚ to achieve recombinant DNA. The gene that is the focus of this
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