BIO1130 - Archean Eon Keywords - Shivan Desai Aerobic: Requiring oxygen to survive‚ and perform life functions. (Aerobe-Organisms that require oxygen for cellular respiration.) Aerobic respiration is a characteristic of eukaryotic cells‚ even though prokaryotic cells can use aerobic respiration as well. Helps produce allot of ATP. Example: Kreb’s Cycle. Anaerobic: Doesn’t require oxygen to survive and perform life functions. (Anaerobe-Organisms that don’t require oxygen to live)
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activity. In this experiment‚ using agarose gel electrophoresis‚ the number and relative positions of restriction sites for three restriction enzymes‚ EcoR1‚ HincII and PvuII‚ on the circular plasmid pBR322 were mapped by determining the length (in base pairs) of the DNA fragments obtained when cutting the plasmid with each of the restriction enzymes separately and each combination thereof. In agarose gel electrophoresis‚ a molecular sieve is created such that the distance traveled in the gel toward
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(require simple growth requirements‚ short generation/ doubling time. (grow rapidly). Easy and cheap to obatain identical cells. Small genome 4.6 million bp for E coli Vector: plasmid‚ virus‚ phage‚ etc (type of vector you use depends on host) DNA source: genomic DNA or cDNA (DNA copied from RNA) Plasmid
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Brianna Noriega In order to analyze a dna molecule in a molecular biology lab you must determine the length in nucleotide pairs. Electrophoresis is an extremely useful tool in order to compare the mobility on agarose gels with dna markers of known lengths. Dna is a polymer that is negatively charged due to the sugar phosphates. When dna is on an electric field such as the electrophoresis gel the different lengths of dna migrate at different rates when they move through the porous gel. The ends
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given protein. However after further research scientists found out that Transformation is not only used to purify protein but also to find out contents that are stored in a given plasmid. The objective of the lab that is to be performed involves a procedure that determines the identity of an unknown gene replicated in a plasmid. To begin this procedure two to four colonies of bacteria is added to two micro tubes filled with CaCl2. In this process the UV light was used periodically to observe the condition
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In the practical sessions template DNA that had the EZH2 (Enhancer of zeste homolog 2) gene already cloned onto it was firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146
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“CLONING OF HORSE RADISHPEROXIDASE GENE IN E.coli FOR EXTRACELLULAR EXPRESSION” Project report submitted to Jawaharlal Nehru Technological University Hyderabad in partial fulfillment for the award of degree of Bachelor of Technology in Biotechnology Submitted by M.RAVI TEJA (08311A2338) & V.MAYURI (08311A2358) Under the Guidance of DR.CHAND PASHA Department of Microbiology Osmania University [pic] Department Of Biotechnology SREENIDHI INSTITUTE OF SCIENCE AND TECHNOLOGY Yamnampet
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So now that we know what recombinant DNA is‚ we are going to look at its purposes. ******** Recombinant DNA is used to insert a gene for production of a protein of interest The production of heat and drought resistant crops can alleviate world hunger around the world. Production of clotting factors can treat bleeding disorders such a Haemophilia which casues bleeding into the joints and can be life threatening. Hepatitis B vaccines are made with the help of yeast cells It is used for the production
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a way to make the bacteria diploid for part of the chromosome. To do this we need to consider a different extrachromosomal element: Ori T The F plasmid (length 105 base pairs) Tra genes There are some special terms to describe the state of F in a cell: F– refers to a strain without any form of F‚ whereas F+ refers to a strain with an F plasmid. F‚ Donor cell Recipient cell F pilus Ori T F is very efficient at transferring itself from an F+ cell to an F– cell. After culturing F+
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genetic material. First a scientist isolates plasmid DNA from bacteria and DNA carrying a gene of interest from cells of another organism‚ such as an animal. A piece of DNA containing the gene is inserted into a plasmid‚ producing recombinant DNA‚ and the recombinant plasmid is returned to a bacterial cell. This cell is then grown in culture forming a clone of cells. The foreign DNA spliced into the plasmid is replicated with the rest of the plasmid as the host cell multiplies. In this way‚ the
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