Ch. 18. viral and bacterial genetics Virus Not living‚ nucleic acids and proteins Viriods and prions Viriods: Single stranded circular Rna Prions: only protein Bacteria Living‚ prokaryotes 1 Seven characteristics common to life Cells and organization Energy use Respond to environmental change Regulation and homeostasis Growth and development Reproduction Change over the course of generations 2 Viruses Over 4‚000 different types of viruses Virus have their
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proteins were taken from bioluminescent jellyfish Aequorea victoria. One of the main lessons of the lab is learning of the use of ‘plasmids’. Plasmids are small pieces of DNA that usually code for one trait and are easily transferable between bacteria. This transfer of plasmids between bacteria is actually extremely helpful for them and are key in their survival. The plasmid that codes for the Green Fluorescent Proteins is accompanied with a gene for resistance to the antibiotic ampicillin. To ‘switch
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be studied in vitro by means of transfection. The success of a transfection essay depends on various conditions. In the experiment the influence of various transfection conditions were studied. An established Rat-2 cell line was transfected with a plasmid containing a mutated Ras gene under various pH conditions. During the focus development stage after successful transfection the cultures developed a fungal infection. Results from previously performed mutual experiments displayed an optimum result
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Home work- Chapter 7: 1. Fertility factors are a. Plasmids that contains genes for antibiotic resistance b. Plasmids that contains genes for pathogenicity c. Plasmids that contains genes for F factor d. All of the above 2. Promoter is a site where‚ a. DNA polymerase binds b. Repressor binds c. RNA polymerase binds d. Inducer binds 3. _______________ enzyme forms RNA based on the information carried on DNA strand. a. DNA polymerase b. RNA polymerase c. DNA helicase d. A & C 4. Operator
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Chitobiase is encoded by chb. In this experiment‚ a restriction map for restriction enzymes Eco R1‚ Pst1 and Hind III using Southern hybridization and restriction analysis of pRSG 192. pRSG 192 is a recombinant plasmid derived from the chb gene and pUC 19‚ a 2.7kb engineered plasmid which encodes for ampicillin resistance‚ a portion of the lac operon and a multiple cloning region . The chb gene exists as a 3.6 kb insert in the mutiple cloning region of pUC 19. The major goals of Experiment
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Microbial Cell Factories BioMed Central Open Access Research Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol Eric J Steen1‚2‚ Rossana Chan1‚3‚ Nilu Prasad1‚3‚ Samuel Myers1‚3‚ Christopher J Petzold1‚3‚ Alyssa Redding1‚3‚ Mario Ouellet1‚3 and Jay D Keasling*1‚2‚3‚4 Address: 1Joint BioEnergy Institute‚ 5885 Hollis Avenue‚ Emeryville‚ CA 94608‚ USA‚ 2Department of Bioengineering‚ University of California‚ Berkeley‚ CA 94720‚ USA‚ 3Physical Biosciences
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Are You a Good or Bad Student? Being a good student is what everyone wants to believe they are. But in reality we all know that there are bad students. I‚ myself would like to believe I am a good student but when I looked over the facts it seems that I am not a bad or a good student. The first and foremost important quality of a good student is‚ of course‚ hard working. We can’t have a good result in academic success without training and effort. The next quality is active in community. A good student
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Recombinant DNA – A DNA molecule made in vitro with segments from different sources. 6. Sticky ends – A single-stranded end of a double-stranded DNA restriction fragment. 7. Cloning vector – An agent used to transfer DNA in genetic engineering. A plasmid that moves recombinant DNA from a test tube back into a cell is an example of a cloning vector‚ as is a virus that transfers recombinant DNA by infection. 8. Nucleic acid hybridization – Base pairing between a gene and a complementary sequence on
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to ligate the pieces of DNA to the vector. Then transform the ligation mixture and the competent cells and create a population with various insert: vector ratio. Any cell that gives rise to a colony has taken up a plasmid and any white colonies has the Vibrio fischeri ligated to plasmid. Glowing colonies have the lux gene ligated to the vector.Isolate and streak a single glowing colony. Use PCR to clone both the glowing colony and the Vibrio fischeri DNA. Sequence the product and finally
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Human Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed
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