Microbiology 215 exam #2 chap 7‚ 11‚ 12 81-90 essay: 10 points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis
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passed to subsequent generations of cells or passed between organisms‚ and how the expression of their info within an organism determines the particular characteristics of that organism. Genome: the cell’s genetic information that includes it’s plasmids and chromosome. Chromosomes: structures containing DNA that physically carry hereditary info‚ contain the genes Genes: segments of DNA (or RNA in the case of viruses) that code for functional products Base pairs: always occur in a specific way
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through a carrier/vector. Plasmids and viruses are commonly used vectors. An enzyme‚ DNA ligase‚ joins the 2 DNA fragments chemically. 7) In gene cloning‚ the bacterial cells take up the recombinant plasmid DNA through a process called transformation. Bacterial cells can be transformed using electric pulsation or heat. The short electric pulse or a brief rise in temperature causes openings in the plasma membrane. The bacterial cells make copies of the recombinant plasmid DNA during cell
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FACT: Potassium tellurite (K2TeO3) inhibits the growth of gram-negative bacteria and most of the upper respiratory tract normal flora. Oxyanions of tellurium‚ like tellurite (TeO32-)‚ are highly toxic for most eukaryotic and prokaryotic organisms. Known for its antimicrobial properties‚ potassium tellurite has been used in selective media for the isolation of a number of naturally tellurite-resistant bacterial species such as the C. diphtheriae. These tellurite-resistant bacteria reduce tellurite
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Scientists can insert the gene that codes for insulin into a bacterial plasmid‚ thus‚ leading the bacteria to produce human insulin. The way in which this is done is that the plasmid is cut by restriction enzymes; leaving behind the cohesive ends. ¡§Special linking sequences are added to the human cDNA so that it will fit precisely into the loose ends of the opened plasmid DNA ring.¡¨ The completed ring‚ called the recombinant plasmid‚ is now inserted into a bacterial cell and allowed to produce insulin
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_______ is a tiny circle of bacterial DNA that is capable of replicating outside of the main bacterial chromosome. plasmid 28. When genes are inserted into lung cells of cystic fibrosis patients‚ this kind of treatment is referred to as _______________. gene therapy 29. Combining the DNA of two different organisms is called ______________ cloning. transgenic 30. Plasmids or viruses can serve as _______________ to carry foreign DNA into the host cell. vectors 31. When the method is
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2013 DESIGNER GENES TRAINING GUIDE by Karen L. Lancour DISCLAIMER - This presentation was prepared using draft rules. There may be some changes in the final copy of the rules. The rules which will be in your Coaches Manual and Student Manuals will be the official rules. • BE SURE TO CHECK THE 2013 EVENT RULES for EVENT PARAMETERS and TOPICS FOR EACH COMPETITION LEVEL TRAINING MATERIALS: • Training Power Point presents an overview of material in the training
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Control‚ Genome and Environment Cellular Control & Variation Cellular Control (a) state that genes code for polypeptides‚ including enzymes; (b) explain the meaning of the term genetic code; The sequence of the bases on a gene is a code with instructions for the construction of proteins. It has a number of characteristics: It is a triplet code- three bases code of an amino acid It is a degenerate code- All amino acids bar one have more than one code Some codes don’t code for amino acids
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What is DNA cloning? DNA cloning is the procedures to produce multiple copies of a single gene or segment of DNA. a DNA fragment containing the gene of interest is isolated from chromosomal DNA using restriction enzymes and then united with a plasmid that has been cut with the same restriction enzymes. When the fragment of chromosomal DNA is joined with its cloning vector in the lab‚ it is called a "recombinant DNA molecule." Following introduction into suitable host cells‚ the recombinant DNA
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Week 2 Quiz Part 1 of 2 - Part I 20.0/ 20.0 Points Question 1 of 12 2.0/ 2.0 Points Which of the following groups of organisms is not prokaryotic? A.Archaea B.Bacteria C.Eubacteria Correct D.Eukarya Answer Key: D Question 2 of 12 2.0/ 2.0 Points The shape of a bacterial cell is determined (and maintained) by the A.cell membrane Correct B.cell wall C.capsule D.slime layer Answer Key: B Question 3 of 12 2.0/ 2.0 Points
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