Chapter 7 * Why do we want to make Eukaryotic cell proteins? * Because when eukaryotic proteins are expressed in prokaryotic cells problems like been unstable‚ no biological activity and prokaryotic contaminants ( pyrogens) can occur. * Also to make the protein as natural as possible * The same expression vectors as we need for pro * We don’t need shangdolron coz that only for pro Transformation: in Eukaryotic cells: in humans means that difference in growth characters
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Blue white selection is a widely used method in screening recombinants in cloning. This is based on the gene product of lac z gene. The plasmid vectors contain this gene which produces β galactosidase enzyme. When a gene is inserted close to lac z gene‚ the reading frame will be distorted and the gene is inactivated. So the transformed cells will not produce this enzyme and are called competent cells. After the recombination‚ the bacterial cells are grown in a medium containing X gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside)
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prokaryotic organisms that lack a true nucleus (i.e.‚ a nucleus defined by a membrane). Bacteria maintain their genetic material‚ deoxyribonucleic acid (DNA)‚ in a single‚ circular chain. Bacteria also contain DNA in small circular molecules termed plasmids. The Dutch merchant and amateur scientist Anton van Leeuwenhoek was the first to observe bacteria and other microorganisms. Using single-lens microscopes of his own design‚ he described bacteria and other microorganisms as "animacules." In addition
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BioLab3 DNA Structure and Function Lab Report Student Name: I. DNA Structure Define the following terms: Purines A colorless crystalline compound with basic properties‚ forming uric acid on oxidation. Pyrimidines A colorless crystalline compound with basic properties; a substituted derivative of this‚ especially the bases thymine and cytosine present in DNA. Nucleotides A compound consisting of a nucleoside linked to
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Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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Cloning plants Many plants clone themselves naturally to reproduce. They send a small shoot-like structure called a runner‚ along the soil. The runner grows into a new separate plant‚ which is genetically identical to the original plant - a clone. People can clone plants by simply taking a cutting of the plant such as a twig or stem and planting it. This is called vegetative propagation. Horticulturists use cloning to grow plants with specific qualities‚ like height‚ flower colour and quality
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Which of the following is most likely to occur when a tumor-suppressor gene is mutated? - The tumor-suppressor gene and resulting protein may lose its function and ability to suppress cell proliferation. Mutations can produce a polypeptide with increased function. - TRUE ________can convert proto-oncogenes into oncogenes. - Nonsense mutations Most human embryos that are aneuploidy - are spontaneously aborted in the first trimester. Horses and donkeys are closely related species that can
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Bio Review – Cells Cell Theory 2.1.1 * All living things are made of cells * Smallest unit of life * Existing cells from pre-existing cells * Contain a blueprint for growth‚ development‚ behavior * Site of all chemical rxns 2.1.2 * Abiogenesis * Living from non-living matter * Robert hook * Compound microscope + observed cork “cells” * Anthony van Leeuwenhoek * Observed blood cells and other “living” cells * Louis Pasteur * Proved
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Transposon-mediated Insertional Mutagenesis in Gene Discovery and Cancer This dissertation is submitted for the degree of Doctor of Philosophy By Jun Kong Team 113‚ Experimental Caner Genetics Group The Wellcome Trust Sanger Institute Wellcome Trust Genome Campus Hinxton‚ Cambridge CB10 1SA Darwin College University of Cambridge Silver Street Cambridge CB3 9EU DECLARATION I hereby declare that this dissertation is the results of my own work and includes nothing which is the outcome of work
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Introduction The energy consumption grows year by year with the economical development of the countries such as China and Japan despite the boisterous events. With the ‘Arab Spring’ in 2011‚ energy market was shook in terms of both production and consumption of the oil with the loss of one of the suppliers‚ also the massive earthquake and tsunami that hit Japan coast caused immediate action for nuclear power and other fuels around the world. Due to those events‚ oil prices reached an all-time record
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