MICROBIOLOGY COURSEWORK The effect of antibiotic concentration on bacterial growth Introduction Null Hypothesis- Increasing the antibiotic concentration has no effect on bacterial growth. Experimental Hypothesis- Increasing the antibiotic concentration decreases bacterial growth. Scientific Theory Bacteria are prokaryotes and can be identified by their shape. Spherical bacteria are called cocci‚ rod shaped bacteria are called bacilli‚ spiral shaped bacteria are called spirilla and
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Biochemical Engineering James M. Lee Department of Chemical Engineering Washington State University Pullman‚ WA 99164-2714 jmlee@wsu.edu Chapter 1. Introduction ................................................................ 1 1.1. 1.2. 1.3. 1.4. 1.5. 1.6. Biotechnology .............................................................................. 1 Biochemical Engineering............................................................. 2 Biological Process....................................
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Study Questions: Chapter 28 1.) Archaea and Bacteria are the oldest‚ structurally simplest and most abundant forms of life. 2.) Name and describe seven ways in which prokaryotes differ substantially from eukaryotes? Unicellularity- fundamentally single-celled Cell Size- can vary (large range changes with species) Nucleoid- lack a membrane-bound nucleus but rather a nucleoid region Cell Division/Genetic Recombination- binary fission (does not use spindle) and do not have a sexual cycle Internal
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Coimisiún na Scrúduithe Stáit Scrúduithe Stáit State Examinations Commission State Examinations Commission Leaving Certificate 2012 Certificate 2012 Marking Scheme Scheme Biology Design and Communication Graphics Higher Level Introduction The marking scheme is a guide to awarding marks to candidates‟ answers. It is a concise and summarised guide and is constructed in a way to minimise its word content. Examiners must conform to this scheme and may not allow marks for answering outside
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generally consists of the following steps: * To isolate a gene‚ we must extract the cell’s DNA. * A gene can be introduced into a cell using a vector. A vector is a vehicle by which a gene is transferred from one cell to another. * *A plasmid can be used as a vector. It is a circular DNA molecule found in some bacteria. * The vector must be extracted from the bacterium before it can be used as a vector. * Now we need to isolate the gene that we want from the DNA. For this we use
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Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) Definition Definition In molecular biology‚ restriction fragment length polymorphism‚ or RFLP is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites‚ and to a related laboratory technique by which these segments can be illustrated. In RFLP analysis
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Why are sexually transmitted diseases more likely to affect females to a greater extent than males? • Sexually transmitted diseases are more likely to affect females to a greater extent than males because they expose a greater surface area of mucous membrane during sexual contact. Why is a woman more likely to get an STD from an infected male than a male is to get it from an infected female? • A woman is more likely to get an STD from an infected male than a male is to get it from an
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the typical size difference between animal cells and bacterial cells? The typical size difference between animal cells and bacterial cells is that animal cells normally are significantly larger. Another difference is that bacterial cell contains a plasmid‚ against the chromosomes that animals possess. Part B: The Gram Stain This procedure is used to separate what two types of bacteria? Gram Stain This separation is based on differences in what physical trait? The separation is based on differences
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exciting experience was where we aimed to induce over-expression of a particular gene in bacteria. We used the polymerase chain reaction technique‚ the gel electrophoresis with agarose followed by isolation of fragments from the gel‚ bacterial plasmid isolation‚ restriction mapping and during class excursions I got to see the use of this techniques on a large scale like the production of antibiotics using the process of fermentation
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Name Microbiology 2210 Answer Key Practice Test 1 Directions: Choose the most correct response to answer the statement. ___1. Archaea a. belong to the kingdom Protista b. have petidoglycan in their cell walls c. belong to the domain Bacteria
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