Restriction Fragment Length Polymorphism (RFLP) Restriction fragment length polymorphism (RFLP) analysis was one of the first techniques to be widely used for detecting variation at the DNA sequence level. The principle behind the technology rests on the possibility of comparing band profiles generated after restriction enzyme digestion in DNA molecules of different individuals. Diverse mutations that might have occurred affect DNA molecules in different ways‚ producing fragments of variable lengths
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To begin the experiment‚ 8 ml of 0.1% Bromophenol blue (BPB) solution was made by diluting 0.25% BPB solution. From the 0.1% BPB solution‚ six diluted solutions‚ ranging from 1:50 to 1:10000‚ were prepared. Each solution was then run in the UV spectrophotometer to measure the absorbance at 590nm. DI water was used as a blank and samples were measured starting from the least concentrated one. The graph of concentration versus absorbance was plotted from the obtained data. In the second part‚ each
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Christal J. Thomas November 7‚ 2010 BCMB 409/ Section 001 Professor: Tom Dockendorff Bacteria: Communication Equals Modification Bacteria are organisms that are extremely copious upon this planet. They are tiny and most are single celled organisms that can survive in just about any environment. Anywhere from plants to the human body is where these organisms can be discovered. Some of the strangest places that support bacterial life include places that have extremes of temperature. These
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B) rRNA C) tRNA D) mRNA E) None of the answers are correct; all of these are products of transcription. Answer: A Skill: Understanding 6) Which of the following statements about bacteriocins is FALSE? A) The genes coding for them are on plasmids. B) They cause food-poisoning symptoms. C) Nisin is a bacteriocin used as a food preservative. D) They can be used
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peritrichous flagella and non-caspulated . Bt belongs to the family of bacteria‚ Bacillus cerus (B. cerus)‚ which produces toxins that cause gastroenteritis (food poisoning) in humans. However‚ Bt is different than B. cerus because it contains a plasmid that produces protein crystals that are toxic to insects instead of causing food poisoning . Indigenous to many environments‚ Bt is found in many terrains such as soils‚ insects‚ stored-product dust and deciduous and coniferous trees . They are also
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Ashley Crane Central Maine Community College Jurassic Park was a very popular film that entertained the notion that scientist could bring back an extinct species with DNA from mosquitos that were carefully preserved in amber. Now‚ a group of scientists are trying to bring back the great passenger pigeon‚ Ectopistes migratorious. According to the Firefly Encyclopedia of Birds‚ there were 3‚000 million passenger pigeons in the late 18th Century‚ making it one of the most abundant species in the world
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DNA replication is a biological process that occurs in all living organisms and copies their DNA. DNA replication during mitosis is the basis for biological inheritance. The process of DNA replication starts when one double-stranded DNA molecule produces two identical copies of the molecule. Each strand of the original double-stranded DNA molecule serves as template for the production of the complementary strand‚ a process referred to as semiconservative replication. Cellular proofreading and error-checking
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Cell: The cell is the basic structural‚ functional and biological unit of all known living organisms. Cells are the smallest unit of life that is classified as a living thing‚ and are often called the "building blocks of life". Discoverer: The cell was discovered by Robert Hooke in 1665. The cell theory‚ first developed in 1839 by Matthias Jakob Schleiden andTheodor Schwann‚ states that all organisms are composed of one or more cells‚ that all cells come from preexisting cells‚ that vital functions
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Biology IB Standard Level 2012-2014 Index Topic 1 Topic 2 Topic 3 Topic 4 Topic 5 Topic 6 Topic 7 Topic 8 Topic 9 Topic 10 Topic 11 Statistical analysis Cells The chemistry of life Genetics Ecology and evolution Human health and physiology Nucleic acids and proteins Cell respiration and photosynthesis Plant science Genetics Human health and physiology Topic
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Microbes‚ despite being the most abundant organisms on Earth‚ were relatively inconspicuous to humans until the 17th century. These life forms have evolved their mechanisms of growth and survival in order to face the harsh conditions of the planet. While it often seems like two types of microbes‚ viruses and bacteria‚ have only impacted human life by increasing the fatality rate‚ Dorothy H. Crawford’s book‚ Deadly Companions‚ refutes this claim. Crawford argues that there are more important effects
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