Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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Criteria 6 8 10 Title Your lab report has a Title that is directly related to the lab experiment/ exercise. Your group or class must be able to come up with your own title You must make your own title based on scientific theory Aim You have clearly stated your aim Your aim is relevant and informative You must make your own aim based on scientific theory Background background information relevant to the lab experiment/ exercise You must be able to link your aim to the background The
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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onto it was firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be
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