Title of the lab: Transformation : Bacterial Genetics Purpose of the lab: The pupose of the lab was to transfor a bacterial E. Coli by using the green flurescent protein from the jellyfish. Another important that was fferdone by making the cell competency‚ meaning that it will be able to take on additional DNA. This was done when the plasma was added. Materials: 1. 37 o C water bath 2. Ice 3. Sterile transfer pipette 4. Foam tube rack 5. Transformation solution (CaCl2) 6. pGLO plasmid 7. Sterile Inoculating
Premium Bacteria Escherichia coli DNA
Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
Premium DNA Gene
In this lab‚ we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid‚ which is relatively small‚ circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism‚ it is absorbed into the organism’s own genetic code. After this occurs‚ the newly introduced gene begins coding for proteins‚
Premium DNA Gene Bacteria
Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
Premium DNA Gene Bacteria
Introduction Transformation is a genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surrounding through the cell membrane. The arabinose operon changes AraC from a repressor to an activator; in this experiment the pGLO plasmid has been designed with a modified operon so that in the presence of the arabinose the bacterial cells which have been transformed by the pGLO plasmid will fluoresce due to the production of GFP. SDS-PAGE
Premium DNA Gene Bacteria
Malak Zomrawi 4/9/15 Bacterial Transformation I. Abstract In the lab‚ the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB‚ ampicillin‚ and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence‚ fluorescence
Premium Bacteria DNA Molecular biology
Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
Premium Bacteria Escherichia coli DNA
understand the process of transformation and the effects on gene expression. The pGLO(-) culture had growth on the LB medium‚ while the LB amp and LB amp + ara mediums had no growth. It was expected that the LB medium had growth on the plate because it served as a control. The LB amp and LB amp + ara had no growth or glow under UV light because they were not successfully transformed and still contained the antibiotic ampicillin that prevented the growth of E. coli (3). The pGLO(+) culture had growth
Premium Escherichia coli Bacteria DNA
Transformation in bacteria is the genotype alteration by the uptake of naked‚ foreign DNA from the environment. This concept of transformation was first discovered when Fred Griffith an experiment using mice and strains of pneumonia. Griffith concluded that a “principle” was transferred from heat-killed S strains to the R strains‚ which transformed them into deadly S strains. Oswald Avery later determined‚ through a series of experiments‚ that DNA was the “principle” that caused the R stains to become
Premium Bacteria DNA Gene
Student Lab Section 6 E. Coli Genetic Transformation with pGLO Plasmid Introduction: Genetic transformation is where one organism takes on a characteristic from another organism (Bacterial Transformation 2013). For this experiment we used the bacteria E. Coli to take in foreign jellyfish DNA which will allow it to change genetic material. This experiment determines the effects that the plasmid pGLO has in transferring the Green Florescent Protein found in a jellyfish into the bacteria. It
Premium Escherichia coli Plasmid Bacteria