9/23/12 Lab Report #1 Meter Reading Summary The objective of this experiment was to learn how to read different meters like the D.C. volt meter and the D.C. amperes meter. In all meters each big line is a major division and each little line in between is a minor division‚ and if there is a line smaller than the minor division lines then that would be a sub minor division. Each meter has a low‚ medium‚ and high range. For example on the D.C. volt meter the ranges go from top to bottom 150‚ 15‚
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Lab Report Diffusion is - One of two kinds of passive transport‚ - Diffusion can transport ions from higher concentration to lower concentration region without any other forces. - A net movement of molecules in and out of cell membrane - Diffusion can be affected by the steepness of the concentration gradient. Lab question: Is the rate of diffusion influenced by the presence of second molecule? Prediction: the rate of diffusion is influenced by the presence of second molecule
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1. The overall goal of this lab was to carry out experiments that clearly demonstrate the different ways DNA genetic information‚ specifically transduction and conjugation. The first half of this experiment focused on exploring the mechanisms of transduction. This was done by creating a spot titer plate for phage carrying kanamycin resistance and E. coli. E. coli was then proven to have gained kanamycin resistance throughout transduction as demonstrated by its ability to grow on a medium containing
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Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
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during the lab session Procedure Part A This lab was performed by the group members as they closed both low and high flow rotameters (Fig-2) and filled the tank with water. Then‚ the large rotameter was opened when the water in the tank had reached the internal baffle‚ so the flow from the tank was reduced by doing so. One member was in charge of adjusting the level of the water so it was always maintained over the baffle’s level during the lab (Fig-1). The first part of the lab was done
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Lab Report Marisa McKinney P. 2 Purpose: To investigate the periodic variation of density in Group 4A elements. Background: When the elements are arranged in order of increasing atomic number‚ they exhibit periodic recurrence of properties. Elements in the same group in the periodic table tend to have similar physical and chemical properties. These similarities are due‚ in large part‚ to similarities among the electron configurations of the elements in a group. You can find periodic
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"18. Dehydration of 2- Methylcyclohexanol." Organic Chemistry Lab 2040L. XU Chemistry‚ n.d. Web. 12 Feb. 2013. 2. "Material Safety Data Sheet 2-Methylcyclohexanol." Http://www.coleparmer.com/Assets/Msds/97403.htm. Coleparmer‚ 19 Mar. 1998. Web. 12 Feb. 2013. 3. "Material Safety Data Sheet Methylcyclohexane." Sciencelab.com. Science Lab‚ 09 Oct. 2005. Web. 12 Feb. 2013. 4. Helmenstine‚ Anne M.‚ Ph.D. "How to Write a Lab Report." About.com Chemistry. About.com Guide‚ n.d. Web. 23 Feb. 2013
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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gene encoding the enzyme lactate dehydrogenase [ldhA] from the bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative
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Crystallization: Lab Report Theory/Introduction The purpose of this lab is to understand the concept of purification through crystallization. Crystallization is the process of purifying a substance‚ with the most adequate solvent. The process of crystallization came to be based on the principle of solubility. First‚ we must choose a solvent. The solvent is crucial to crystallization because it’s what will allow impurities to dissolve. Therefore‚ when selecting a solvent it is important that it
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