DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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tails form a barrier to most water soluble substances. Membrane proteins serves as channels for transport of metabolites‚ some act as enzymes or carriers‚ while some are receptors. Lastly carbohydrate molecules of the membrane are relatively short-chain polysaccharides‚ which has multiple functions‚ for example‚ cell-cell recognition and acting as receptor sites for chemical signals. The plasma membrane is a permeable membrane. An important role of the plasma membrane is to maintain the integrity
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Title: The affects of hydrogen peroxide on catalase reactions in animal and plant cells at different temperatures and states. Introduction: All living organisms in the kingdoms of life are composed of and depend on cells to function normally. Not all cells‚ however‚ are alike. There are two primary types of cells: eukaryotic and prokaryotic cells. Cells contain organelles‚ or tiny cellular structures‚ that carry out specific functions necessary for normal cellular operation. (Regina Bailey Updated
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too long (shriveling is a common misconception)‚ a tea bag’s contents diffuse through the water. These are all examples of diffusion and osmosis. These phenomena happen everyday and people don’t realize what is actually happening. The purpose of the lab is to see the effects of diffusion and osmosis. In part B‚ the higher the concentration of solute‚ the higher the mass of the solution. Methods and Materials: For part A of the experiment‚ the following items are needed: Dialysis tubing‚ IKI solution
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------------------------------------------------- SIMULATION REPORT The objective of the project was to trade on a Market Simulation “STOCK TRACK”‚ from 2/13 to 4/20. We were supposed to try to trade different types of instruments in order to gain more knowledge about they are trade in the real world; and how a strategy which seems good can be overturned by the market circumstances. During this period as a conservative‚ I tried to trade less risky instruments. The qualities that I used to
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Writing Practical Reports Aim: To observe what happens during and after a chemical reaction. Equipment: Concentrated nitric acid in a glass dropping bottle Small pieces of Copper 250ml Beaker 2 M Lead nitrate in a dropping bottle 2 M Potassium iodide in a dropping bottle 2 M Copper sulfate in a dropping bottle 2 M Sodium hydroxide in a dropping bottle 2 M Hydrochloric acid in a dropping bottle 4 Pyrex test tubes Test tube rack Spatula Bunsen burner‚ gauze
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causing the body to not have enough acids in the urine. These factors are all very different‚ but are interconnected because an imbalance in any of these factors can lead to imbalances in other areas as well (Wise‚ 2010). The importance of this lab is to analyze factors that may affect urine concentration‚ pH‚ and rate of production (flow rate) though multiple scenarios over a period of 60 minutes. My hypothesis for the urine analysis experiment is that the urine volumes will increase because
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affecting the rate of reaction‚ PNPP (p-nitrophenyl phosphate) + H20 ? PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase‚ which alters its active site‚ and thus changes its efficiency to catalyze the reaction. We measured the rate of reaction‚ by using a chromogenic
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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Lab Ex#8: "Enzymes: Catalysts of Life" INTRODUCTION Enzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity‚ we would not possess the catalyst activity necessary for energy to produce movement. Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables‚ such as temperature
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