the gel after electrophoresis. Gel 1 Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis‚ both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it‚ to find out if we are homozygous or heterozygous. Discussion For this lab‚ DNA from our cheek cells were separated through PCR‚ and singled out through gel electrophoresis
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Gel Electrophoresis Lab SBI4U1 May 13th‚ 2013 Gel Electrophoresis Lab Purpose: The purpose of this lab is to learn how restriction enzymes cut DNA molecules at specific sequences‚ thus producing DNA fragments of various lengths. Students learn how fragments form unique patterns‚ which help to distinguish the base for DNA identification. This lab answers the question “whose DNA was left behind?”. Materials: * Transfer pipets * Agarose Gel * Dyed DNA samples *
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Bernardine Date Due: 02/08/2013 PURISIMA‚ Dio Mark Angelo Date Submitted: 02/08/2013 Experiment No. 9 AGAROSE GEL ELECTROPHORESIS OF DNA Abstract _____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more
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Gel Electrophoresis is used to separate the haemoglobin component of blood. Because each type of haemoglobin (HbA‚ HbS‚ Hbc and more) have different electrical charges‚ they will separate after undergoing gel electrophoresis. Firstly‚ a blood sample from the patient is taken and is applied to a cellulose acetate membrane strip that has been soaked in a buffer solution along with saponin. The red blood cells are lysed by the saponin while being soaked‚ therefore they release their haemoglobin. A
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this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase
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The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
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Function 16 October 2014 Gel Filtration and Electrophoresis Objective The essential goal of the experiment was to separate proteins in a solution based on size in different fractions. The relative protein content for each one fraction was found through the utilization of an amido black-based protein assay. Later in the trial polyacrylamide gel electrophoresis was utilized to separate BSA from hemoglobin. Methods I. Gel Filtration and Protein Assay: 1. A slurry of Bio-Gel P-100 beads in water was
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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Gel Electrophoresis Adventure Intro The final goal of this lab was to successfully measure the size of different samples of DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. The DNA samples will move through the gel towards the positive charge. Ideally‚ the DNA will move and create and sequence of smallest to largest. This lab exposes us to DNA technology. Backround Gel electrophoresis is used to separate macromolecules like DNA or RNA by size or
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steps: • Denaturation: Samples of DNA with the target sequence are heated to a reaction temperature of 94-96˚ C that causes DNA melting of the DNA template without denaturing the enzyme by disrupting the hydrogen bonds between complementary bases of the double helical DNA‚ yielding single-stranded DNA molecules(“How Is PCR (polymerase Chain Reaction) Done?”). DNA polymerase does not get degraded in such high temperatures since the DNA polymerase used in this reaction is thermostable as it is isolated
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