BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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Abstract For this lab the rate of photosynthesis was analyzed upon plant leaves. A sample of a light exposed and a not-light exposed leave were used to clearly identify the role of sunlight in the process of producing and storing energy. Aim We are trying to find proof‚ to demonstrate the necessity of light and chlorophyll in the process of photosynthesis. Background Photosynthesis occurs in organisms which contain chlorophyll. It’s a process that involves the chloroplasts to synthesize glucose
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was to take different food samples and use a device known as a calorimeter made from a can of aluminum‚ one large paper clips and a string of copper wire to measure the amount of thermal energy released during a reaction. By measuring the temperature difference before and after a reaction‚ the calorimeter indicates how much energy is released. From personal experiences I had previously learned about the release of energy from different types of food samples. For example‚ from a previous class we
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the limiting reactant of a chemical reaction that involves iron filings and a copper sulfate solution. Second‚ to determine the mole ratios between iron and copper and to show that a single replacement reaction occurs when iron and copper sulfate react with each other. And lastly‚ to determine/calculate the percent yield to better analyze the chemical reaction between iron and copper sulfate to produce copper and iron sulfate. The method used to perform this lab will be to combine a metal with a solution
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Reactions in Aqueous Solutions: Metathesis Reactions and Net Ionic Equations Introduction: Metathesis or double decomposition reactions are a reaction in which two compounds react to form two new compounds‚ with no changes in oxidation number. The ions of two compounds exchange partners. AX + BY AY + BX This reaction can occur between two inorganic salts when one product is insoluble in water‚ driving the reaction forward. A typical example is as followed and is considered a molecular equation
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Abstract This article is investing the effects of speed of the action potential across many neurons through investigating two diseases and performing related lab simulations. Multiple sclerosis and epilepsy are the two disease which are investigated and through the use of Neurons in Action lab simulations‚ we saw the effects that demyelination and channelopathy can have. As my hypothesis guessed‚ demyelination is the main cause of multiple sclerosis and channelopathy is the main cause of epilepsy
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Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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purpose of this lab was to find the theoretical and experimental percentage yields of the double displacement reaction between the solutions Lead (II) Nitrate (PbNO3) and Potassium Iodide (KI). It is important to obtain amounts of Lead (II) Nitrate and Potassium Iodide as close to 1.44g as possible. This reaction creates Lead (II) Iodide and Potassium Nitrate. The precipitate during this reaction is Lead (II) Iodide. The balanced equation is Pb(NO3)2 + 2KI= PbI2 + 2KNO3. In this lab the Lead (II) Iodide
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