Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
Premium DNA Molecular biology Escherichia coli
Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells can
Premium DNA
an ideal form in which to store glucose molecules and these can be reformed by hydrolysis later when needed. Starch is a mixture of two types of chain (amylose and amylopectin) formed from alpha-glucose. Amylose is formed when many alpha-glucose molecules condense together forming 1‚ 4 glycosdic bonds‚ so producing long unbranched chains. These chains take up a coiled or helical configuration as each monomer has a bulky side group which has to be accommodated. Amylose forms about 20% of starch.
Premium Starch Iodine
Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing the most amount
Premium DNA Bacteria Escherichia coli
Light Reactions Lab This lab explores the effect of light intensity and light wavelength on photosynthesis. The quantity of energy [ATP] produced will change depending upon on these parameters. Use "Johnson Explorations: Photosynthesis" located at http://www.mhhe.com/biosci/genbio/biolink/j_explorations/ch09expl.htm Examine the diagram before clicking "skip intro" so you have an idea what is going on. The animation show the movement of photons‚ electrons‚ water‚ protons‚ ATP etc. relating to
Premium Photosynthesis Light Oxygen
Our first task was to study the market research and decide our initial strategy. To decide our initial strategy‚ first we analyzed the different types of research data. There were five segments to choose from; Cost cutter‚ Work horse‚ Innovator‚ Mercedes and Traveler. We reviewed the customer needs for each segment and the information regarding how customers intend to use computers. After that we looked at the average price each segment was willing to pay for the ideal brand in different cities
Premium Marketing Brand management
Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions
Premium Genetics DNA Gene
Round-Trip Copper Reactions Lab The purpose of this lab was to evaluate our skills of decanting a supernatant liquid without losing the solid and successful completion of a series of reactions. This was done through five chemical reactions involving copper. In this lab‚ elemental copper was put through five different chemical reactions in order to convert it into different compounds. By the end of the fifth reaction‚ the copper was back to its elemental state. In the first reaction‚ 0.95 g of pure
Premium Sulfuric acid Copper Chemistry
1.05 assessment by ??????? 1) What does it mean when scientists say that living organisms share a universal genetic code? Genetic code means DNA and when organisms share a univeral genetic code they share a structure of DNA. 2) How does a universal genetic code relate to the hypotheses about the origin of life on Earth?Universal Genetic code would indicate that all organisms descended from a common ancestor‚ seeing how all organsims share a
Premium DNA Gene
Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
Premium DNA Bacteria Molecular biology