Department of biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris LABORATORY REPORT SBT 1013: INTRODUCTION TO BIOTECHNOLOGY Semester 4 Sessions 2013/2014 ID NUMBER AND NAME 1. SITI NURFATINI BINTI MOHAMAD ROSLAN (E20131007560) 2.NURHAFIZAH BINTI SAMSUDDIN (E20131007584) 3.MUNIRAH BINTI HARON (E20131007556) 4.NORSYAWANI BINTI SUPIAN (E20131007577) LECTURER : CIK FATIMAH EXPERIMENT NO. : EXPERIMENT NO.1 TITLE : EXTRACTION OF DNA DATE
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Lab Report 1 Introduction: Proper chemical formulas entitle many challenges such as the Law of Multiple proportions that states that there may be more than one plausible mole ratio for the elements in that compound. However if we determine the mass of each element in the compound we will be able to get the true chemical formula. In this experiment‚ we used the law of definite proportions to find the chemical formula for a hydrated compound containing copper‚ chlorine‚ and water molecules
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3 March 8‚ 2013 Lab Report: Empirical Formula of Zinc Chloride (ZnCl) * Purpose The purpose of this experiment was to learn how to determine the empirical formula. Empirical means “based on experimental evidence.” * Experimental Design The reaction that occurred was the reaction of the elements Zinc (Zn) and Chloride (Cl) by mixing a piece(s) of Zinc and 50mL of Hydrochloric Acid (HCl). The amount of Zinc was determined to be between 1.00g and 1.25g. As the reaction occurred there was
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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involved in the identification of restriction fragment length polymorphisms. Restrictions were carried out using three different restriction enzymes‚ ECORI‚ HindIII and BstELI with their buffers. Lambda (λ) DNA was then examined using electrophoresis and Southern blotting. The results showed that λ DNA was best digested by EcoRI as all of the expected bands can be seen and are in the right place. The HindIII digestion was second best as there was a large amount of merging and the bands contained a lot
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Assignemnt 2 Problem 3-3 Model 3-1 was has been run for 8 hours (480 minutes) instead of 20 minutes. The plots on queue length and the number of times the drill press is busy or idle for the 8-hour run is given figure 1. Figure 1 (Simple Processing System with 8 hours of time) The primary performance related results are compared in table 1. Table 1: Performance Measures | 20 minutes run | 480 minutes run (8 hours) | Total Production | 5 | 89 | Avg. Wait Time | 2.52 | 3.33 | Avg
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Chemistry Lab Report Water Hydration Introduction: A hydrated crystal or hydrated occurs when water becomes tightly attracted to a metal salt base on it’s polarity. The water molecules maintain integrity as molecules‚ however they are considered to be part of the formula of the hydrate. When the hydrate metal salt crystal is heated‚ the attractions to the water are broken by the heat energy and the water escape from the crystal. After heating the salt crystal is called as anhydrous‚ which
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