Effect of Enzyme and Substrate Concentration on Reaction Rate by Zachary A. Poché Biology 155 Laboratory October 15‚ 2014 Lab Partners: Cade White‚ Hannah Ragas‚ Russheka Aremillion ABSTRACT In order to increase the reaction rate‚ substrates attach to the active site of enzymes which decrease the activation energy required to convert substrates to products. We examined the effect of enzyme concentration and substrate concentration on the overall rate of the reaction. To determine
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INTRODUCTION Many infectious diseases caused by infectious agents‚ including bacteria‚ fungi‚ viruses and parasites‚ have plagued human existence CITATION Kon97 \l 1033 (Koneman‚ 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate
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there is no report on the optimization of polyphenols and their radical scavenging activities from the edible leaves of A. gangetica. Therefore‚ the main aim of this study is to optimize extraction conditions such as EtOH concentration‚ liquid-to-solid ratio and extraction time to obtain the maximum extraction yield‚ phenolic content‚ flavonoid content and DPPH and ABTS radical scavenging activity from the A. gangetica leaves. Furthermore‚ the polyphenols present in the optimized
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TEA Dr. B.L. Wedzicha of t h e Procter D e p a r t m e n t of Food Science‚ U n i v e r s i t y of Leeds‚ describes t h e c h e m i s t r y of black t e a manufacture Tea is the m o s t w i d e l y c o n s u m e d beverage in the w o r l d . The economic importance of an annual w o r l d production of tea estimated to be in the region of 1-1·5 million tonnes has resulted in considerable attention being paid to the understanding of the chemical and physical changes w h i c h take place during tea
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REVIEW OF RELATED LITERATURE The bilimbi‚ Averrhoa bilimbi‚ L.‚ (Oxalidaceae)‚ is closely alliedto the carambola but quite different in appearance‚ manner of fruiting‚flavor and uses. The only strictly English names are "cucumber tree"and "tree sorrel"‚ bestowed by the British in colonial times. "Bilimbi" isthe common name in India and has become widely used.Bilimbisare all much the same wherever they are grown‚ but P.J.Wester reported that a form with sweet fruits had been discovered inthe Philippines
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were gram positive cocci arranged in irregular clusters. I ran a catalase test on both organisms; organism A was catalase positive while organism B was catalase negative. I also performed a cytochrome oxidase test on both organisms; again‚ organism A was oxidase positive while organism B was oxidase negative. Then I decided I would run the biochemical tests on organism B. I observed that organism B was non-motile and did not produce endospores. With these results‚ I narrowed down the possible species
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ABSTRACT In todays world of ever evolving genetic mutations the incidence of drug resistant forms of microorganisms are on the rise. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine
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Medical Microbiology II - Laboratory Report 1 Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral
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Vitamin Description An organic chemical compound (or related set of compounds) is called a vitamin when it cannot be synthesized in sufficient quantities by an organism‚ and must be obtained from the diet. Thus‚ the term is conditional both on the circumstances and on the particular organism. For example‚ ascorbic acid (vitamin C) is a vitamin for humans‚ but not for most other animals‚ and biotin and vitamin D are required in the human diet only in certain circumstances. By convention‚ the term vitamin does
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Large scale Enzyme applications 1. Detergents Bacterial proteinases are still the most important detergent enzymes. Lipases decompose fats into more water-soluble compounds. Amylases are used in detergents to remove starch based stains. 2. Starch hydrolysis and fructose production The use of starch degrading enzymes was the first large scale application of microbial enzymes in food industry. Mainly two enzymes carry out conversion of starch to glucose: alpha-amylase and fungal enzymes
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