record the color intensity in each test tube. Time (min) Tube 1 with catechol Tube 2 with hydroquinone 0 1 1 5 3 1 10 5 4 Based upon color intensity‚ to what degree does catechol oxidase react with hydroquinone? 4 What difference in chemical structure of the two substrates determines the specificity of catechol oxidase? The different location of the second -OH group III. Temperature and Enzyme Activity EXERCISE 3 – Enzyme activity and temperature Record the relative color intensity at intervals
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relieve inflamed joints. Its leaves have polyphenols and flavonoids in which it have the presence of proanthocyanidine in various forms like apigenin‚ anthocyanin‚ procyanidine‚ catechin‚ epicatechin‚ along with taxifolin‚ eriodictyol and naringenin. Other constituents like tartaric acid‚ mucilage‚ pectin‚ arabinose‚ xylose‚ galactose‚ glucose‚ uronic acid and triterpenes have also been identified in Tamarind. Out of these phytoconstituents‚ polyphenols and flavonoids have been well known to exhibit
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E. LOOK AT JOURNAL ARTICLES FOR AN EXAMPLE) ALL ASSAYS MUST BE INCLUDED TO RECEIVE FULL CREDIT (Growth curve‚ CFUs (counting colonies/dilutions‚ can include equation)‚ Growth after incubating at RT for 6 days‚ MIC (antimicrobial disc)‚ Catalase‚ Oxidase‚ DNA isolation). Under each headline describe the methods and material. The example below includes for headline that should be included but not all. ALL ASSAYS MUST BE INCLUDED TO RECEIVE FULL CREDIT and MAKE SURE YOU READ YOUR PROTOCOLS I GAVE TO
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Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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and Results (Experimental) 1 Milkish white/ milkish white Control To control for color change of the mitochondrial enzymes alone without addition of NADI. (false +) 2 Milkish white/ Bright pink to bluish purple Experiment Assay for Cytochrome oxidase activity. (+ purple) Shows the effects from the addition of NADI‚ a very
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unknown: 1 Colony Appearance 2 Motility 3 Gram Stain 4 Thyoglycollate aerobic vs. anaerobic 5 Innoculate T soy slant 6 Optimum growth temperature 37o vs. 42o 7 Nitrate test 8 Methyl Red 9 Carbohydrate fermentation 10 Catalase Test 11 Oxidase test
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Lab 6.C Hypothesis: If enzyme activity is affected by the pH of a solution‚ then the enzymes will experience the greatest activity at a pH of 6. Variables: Independent Variables Dependent Variable Controls Four different pH values (10‚ 7‚ 6‚ and 3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5
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Next‚ an oxidase test was performed to eliminate one or more of the bacterium choices. P. aeruginosa was eliminated as the identity of the bacterium when the oxidase test results were negative. An EMB plate of the bacteria was examined to identify whether the bacterium was S. typhi or E. coli. The growth on the EMB plate was shiny and metallic
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5cm‚ 6cm‚ at the bottom of each test tube. 3. Put your test tubes to the location of the phosphate buffer series and fill each tube according to the following direction. 4. Continue your work area and add 1cm of potato extract obtaining catechol oxidase to each of the seven tubes. 5. Add 1% catechol to each of the 7 test tubes‚ bringing the total volume to the 6c mark. Agitate the contents of the tubes using a vortex mixer if available. 6. At time 0‚ record the relative color intensity of each
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Name: Madison Gullatt Sect/Table: !/D Experiment 8 Report: Extracellular Enzymes Purpose: The purpose of this experiment is to perform a variety of different test and record the data from each test in relation to extracellular enzymes. Results: 1. Fill in the following tables with your observations. Note if the test is positive or negative for the enzyme. Also note the appearance of the colonies and/or media. This will include: the color of the medium immediately surrounding
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