Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA is a polymer‚ which is a chemical compound or a mixture of compounds consisting of repeating structural units. These repeating structures are created through polymerization. The monomer‚ meaning one part‚ units of DNA are nucleotides. Each nucleotide consists of a five carbon sugar‚ also known as deoxyribose‚ and nitrogen containing base attached to the sugar
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and the discovery of DNA Ralf Dahm* Max Planck Institute for Developmental Biology‚ Department 3 – Genetics‚ Spemannstr. 35/III‚ D-72076 Tubingen‚ Germany ¨ Received for publication 5 October 2004‚ revised 17 November 2004‚ accepted 20 November 2004 Available online 21 December 2004 Abstract Over the past 60 years‚ DNA has risen from being an obscure molecule with presumed accessory or structural functions inside the nucleus to the icon of modern bioscience. The story of DNA often seems to begin
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life (1). Significant developments have been made in gene monitoring techniques specifically in DNA microarrays which only very recently revolutionized genome expression analysis (1). Despite continuous improvements and modification to the technique‚ DNA microarrays are still no more than a glass microscope slide studded with individual immobile nucleotide fragments (1‚ 2). The fundamentals of DNA microarrays are set on complementary base-pairing (3)‚ and because the exact sequence and position
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Much can be learned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment‚ you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh‚ Soft Fruit (e.g.‚ Grapes‚ Strawberries‚ Banana‚ etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou
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DNA‚ or deoxyribonucleic acid‚ is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA)‚ but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA). The information in DNA is stored as a code made up of four chemical bases: adenine (A)‚ guanine (G)‚ cytosine (C)‚ and thymine (T). The order‚ or sequence‚ of
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DNA Packaging: Nucleosomes and Chromatin By: Anthony T. Annunziato‚ Ph.D. (Biology Department‚ Boston College) © 2008 Nature Education Citation: Annunziato‚ A. (2008) DNA packaging: Nucleosomes and chromatin. Nature Education 1(1) Each of us has enough DNA to reach from here to the sun and back‚ more than 300 times. How is all of that DNA packaged so tightly into chromosomes and squeezed into a tiny nucleus? The haploid human genome contains approximately 3 billion base pairs of DNA packaged
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DNA fingerprinting is a way of identifying a specific individual‚ rather than simply identifying a species or some particular trait. It is also known as genetic fingerprinting or DNA profiling. As a technology‚ it has been around since at least 1985‚ when it was announced by its inventor‚ Sir Alec Jeffreys. DNA fingerprinting is currently used both for identifying paternity or maternity and for identifying criminals or victims. There is discussion of using DNA fingerprinting as a sort of personal
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chromosomes are very long compact coils of DNA (Deoxyribonucleic Acid) that store all the information that the body inbeds such as how one looks and functions. This paper will first describe the structure of DNA; second discuss how the structure of DNA allows it to serve as the basis for inheritance‚ third examine how meiosis allows DNA to be divided into gametes and finally‚ describe how this relates to Gregor Mendel’s patterns of inheritance. The structure of DNA DNA is a thread formed by two strands
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ions -DNA replication minimizes errors such as the DNA that is duplicated but changes in the DNA do occur‚ producing mutations. Although most mutations are either neutral or harmful they are also the raw material for evolution. Such mutations from alleles‚ alternate forms of a given gene that may produce differences in structure or function such as black‚ brown or blond hair in humans‚ or different mating calls in frogs. Stages of Mitosis~ 1)Parent cell. 2)Chromosomes make identical copies
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