The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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Keegan Bauman Potato Lab Dr. Peano Block 1 Introduction: Potatoes are just your average food; they have been around for as long as the human race can remember. In this lab we will make them be a prime example of osmosis‚ which is a transport mechanism. In this lab the problem being tested was what environment affects the mass of the potatoes the most. The independent variable is the environments used salt‚ water and iodine solution. The dependent variable is the mass of the potato after being
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concentration. Hypotonic solution is the solution with the lower salt concentration. My hypothesis is that the potato core will increase in size. When the solution is hypertonic‚ the potato will decrease in size making the potato hypotonic. Osmosis is really interesting‚ that is why I chose to write this report about it. Materials and Methods We first cut out six cores out of a potato. We trimmed them all to the same length using a razor. After we had cut them to the same size we weighed them using
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Potato Osmosis Introduction: A shipwrecked sailor is stranded on a small desert island with no fresh water to drink. They know they could last without food for up to a month‚ but if they didn’t have water to drink they will be dead within a week. Hoping to postpone the inevitable‚ their thirst drives them to drink the salty seawater. They are dead in two days. Why do you think drinking seawater killed the sailor faster than not drinking any water at all? Today we explore the cause of the sailor’s
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Introduction: In this experiment‚ you will study osmosis. Your task is to design an experiment that will determine what concentration of solute is isotonic with the specimen cells. (Design) Procedure: You will be provided with the following materials: Potato‚ potato corer‚ scalpel‚ forceps‚ electronic balance 10 % salt solution‚ distilled water‚ 50 mL beakers‚ graduated cylinder‚ paper towel You must design a procedure that will enable you to determine the concentration
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Potato Cell Cytoplasm Composition PURPOSE: To determine the concentration of salt in the cytoplasm of potato cells HYPOTHESIS: Increase in salt concentration results in a hypotonic solution where the mass of the potato increases due to the movement of water into the cell. On the other hand‚ decrease in salt concentration results in a hypertonic solution where the mass of the potato decreases due to the movement of water outside the cell. MATERIALS: Potato Forceps Stopwatch Scalpel Test
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without them the reactions within the cells would occur to slow to sustain life. However there are many factors which affect the rate of enzymes reactions. One of them is pH‚ which influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment. RESEARCH QUESTIONWhat is the influence of different values of pH on the activity of catalase in potato tuber cells?VARIABLESIndependent: potato discs treatment (placing them into
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STRUCTURE AND FUNCTION OF LIVING CELLS Name Answer the following questions as you work your way through the lab material typing in your answers. Then submit your completed lab report through the “Assignment” feature. This lab report is worth 50 points towards your final lab grade. Also‚ per the Honor Code‚ this work must be your own. CELLS Using your Biology Textbook answer the following questions. 1. What types of organisms are Prokaryotes? The Prokaryotes are organisms that are
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using potato cores and sucrose solution 1.0 Abstract This experiment’s ultimate goal is to find the water potential of the potato cell. This was achieved through placing potato cores in different concentrations of sucrose (0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%‚ 2.0%‚ 3.0% and 4.0%) solution and to observe how much water was gained or lost through osmosis to reach a prediction of the concentration within the potato cell. The results displayed that the concentration of sucrose within the potato cell is 1
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experiment is to study how enzyme activity is affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in acidic pH levels‚ the absorbency
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