University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
In fermentation the pyruvate is converted into ethyl alcohol‚ through the oxidation of the 2 NADH molecules‚ which returns them to two NAD+’s (Freeman‚ 2011). Oxidation is the loss of an electron in this case H+. We used information from previous labs in which we tested
Premium Ethanol Yeast Carbon dioxide
Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
Premium Antibody Immune system Chemistry
for human erythrocyte behavior because permeability similarity increases for more closely related species (Hunter 1976). Using an even more closely related species’ erythrocytes in diffusion experiments would provide an even better model for human cell behavior. Additionally‚ finding out more about diffusion mechanisms may allow scientists to synthesize compounds that have specific desired diffusion characteristics. This will allow better targeting of drugs‚ which would have a great impact on medicine
Premium Diffusion Chemistry Osmosis
Introduction This report will investigate the six essential nutrients required in our diet. The report will provide the function of each nutrient and explore why they are vital for the body. Carbohydrates Carbohydrates are the most important nutrient for the body as it is the largest possible source of energy. Carbohydrate supplies the body with energy‚ which allows the brain to function more effectively and allows organs to operate sufficiently. When the body has energy this allows each
Premium Nutrition Metabolism Carbohydrate
tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure: 1) Preeminently‚ follow all laboratory safety guidelines ensuring that you have safety goggles on at all times throughout the lab. 2) Fill the cuvette 2/3 of the way with water. 3) Select the appropriate wavelength on the spectrophotometer. 4) Place the cuvette in the spectrophotometer and click the calibrate button. 5) Put 5mL of stock solution into test tube. 6) Fill
Premium Absorbance Laboratory glassware Coffee
Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
Premium Food coloring Color E number
Student Name: Amaan Rushdi Lab Partner Name: Dylan Course: BIOL 123 Lab Instructor: Megan Grandinetti Sowbug behavior on environment -lab report What area does the sowbugs prefer to choose for their dwelling? Abstract By working with physical isopods‚ ordinarily known as pill bugs‚ sow bugs or roly- polies‚ we tried whether these sowbugs favored a dark spot to a light place. Included in the lab are a few outlines and tables sketching out our aftereffects of the test. We measured recurrence
Premium Woodlouse Crustacean Experiment
Created By: Andrew Weeldreyer Mrs. Wolf Period:3 AP Chem Lab Report: Determination of the Molar Volume of a Gas Objective: To react Mg and HCL and form hydrogen gas‚ then after collecting lab data‚ determine the hydrogen gas’s molar volume at STP through calculation. Materials: -LabQuest data collector - 3.0 M of HCl solution - Ribbon of Mg metal - Gas pressure sensor
Free Pressure Ideal gas law Gas
Alka-Seltzer Rocket Formal Lab Report Abstract In the Alka-Seltzer Rocket lab one was to experimentally determine how much (grams) Alka-Seltzer and water in mL‚ is needed to launch the rocket the highest/longest distance in cm. Also to experimentally determine the % NaHCO3 in the tablet using the information from the highest/longest distance. Calculate the error and % error of NaHCO3 in an Alka-Seltzer tablet and to determine the limiting reagent for the highest/longest distance traveled. Alka-Seltzer
Free Acetic acid Sodium bicarbonate Vinegar