Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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of the cell membrane with the correct terms integral proteins‚ channel proteins and bilipid construction. Your last paragraph was very detailed with great information about the cell membrane‚ you stated “ The cell membrane is very thin and fragile‚ and it contains two different types of proteins. “Integral proteins are part of the membrane structure and cannot be removed without damaging or destroying the membrane. Peripheral proteins are bound to the inner or outer surface of the membrane and are
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The main purpose of this lab is to explain what emulsifiers and emulsions are and how emulsifiers affect emulsion stability. An emulsion is a mix of to or more liquids that usually do not mix together. One liquid is evenly dispersed in small droplets throughout the other liquid. Many emulsions contain water as one of the phases. There are two different types of emulsions. One type of emulsion contains oil droplets that are dispersed within water. It is considered oil in water emulsion. The other
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reactions in animal and plant cells at different temperatures and states. Introduction: All living organisms in the kingdoms of life are composed of and depend on cells to function normally. Not all cells‚ however‚ are alike. There are two primary types of cells: eukaryotic and prokaryotic cells. Cells contain organelles‚ or tiny cellular structures‚ that carry out specific functions necessary for normal cellular operation. (Regina Bailey Updated April 06‚ 2018) The cell is needed for survival and
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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concentrations on the rate of osmosis. Artificial cells were filled with different solute concentrations and placed in water and weighed at equal time intervals to show how the water moves across cell membranes and down its concentration gradient into the lower concentrated area. The weights of the cells were recorded each interval‚ and then the rate of osmosis was found by calculating the corrected cumulative change in weight. The prediction made was that the cells with the higher solute concentrations would
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chemical reactions that take place in cells. Enzymes can be found in both liver and potato. They provide a site where reactants can be brought together to react. These reactants are called substrates that fin into the enzyme. The changing of heat‚ surface area‚ chemicals‚ and pH will denature the shape of the enzyme‚ not allowing it to do its function. Testing on peroxidase- an enzyme- will be done on an animal enzyme (chicken liver) and a plant enzyme (potato). For testing Hydrogen Peroxide (H2O2)
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Brianna Cooper Brandon Boucher 2/16/12 Purpose: To understand how both voltaic and electrolytic cells function. Procedure: Refer to pages 34-36 of laboratory notebook for experimental details Results and Discussion: Voltaic Cell Reactions | | | Overall Cell Reaction | Observed Voltage | Theoretical Voltage | Cu2+(aq)+Zn(s) Cu(s)+Zn2+(aq) | 0.947 V | 1.10 V | Cu2+(aq)+Sn(s)Cu(s)+Sn2+(aq) | 0.571 V | 0.473 V | Cu2+(aq)+Fe(s) Cu(s)+Fe2+(aq) | 0.512 V | 0.777 V | Cu2+(aq)+Mg(s) Cu(s)+Mg2+(aq)
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Introduction Memory is defined as the faculty by which the mind stores and remembers information(Eysenck & keane‚ 2010). Atkinson and Shiffrin (1968) (Cowan‚ Rouder‚ & Stadler‚ 2000)‚ came up with a model of sensory memory which stated not everything we perceive we process. Craik and Tulving (1975) developed an opposing theory in which they proposed the levels of processing theory‚ stating its not the processing but the type of quality of the processing that is performed‚ that determines the retention
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