effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is the bodies normal
Free Enzyme Starch Glucose
reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three
Premium Enzyme Catalysis Alkaline phosphatase
Keegan Bauman Potato Lab Dr. Peano Block 1 Introduction: Potatoes are just your average food; they have been around for as long as the human race can remember. In this lab we will make them be a prime example of osmosis‚ which is a transport mechanism. In this lab the problem being tested was what environment affects the mass of the potatoes the most. The independent variable is the environments used salt‚ water and iodine solution. The dependent variable is the mass of the potato after being
Premium Water Potato Environment
Pg1Pg1 SCIENCE EXPERIMENTS ON FILE ™? Revised Edition 5.40-1 Potato Battery Lois Fruen and Chelen Johnson Topic Construction of a battery Time 45 minutes to 1 hour Safety ! Please click on the safety icon to view the safety precautions. Adult supervision is necessary for the soldering of the alligator
Premium Battery Chemistry Experiment
EXPERIMENT TO SHOW THE EFFECT OF DIFFERENT CONCENTRATION OF GLUCOSE ON POTATO STRIPS INTRODUCTION: Molecules of liquid and gas are constantly in motion‚ they move randomly in all directions and bounce around in all directions and bounce around and into each other. As they move‚ they tend to spread out moving from areas with many molecules to areas with fewer molecules . This process of spreading out is called Diffusion‚ for example smell of cooking travelling around the house from the kitchen
Premium Osmosis Concentration Cell wall
LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount
Premium Enzyme PH Temperature
Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
Free Enzyme Amino acid Functional group
Enzyme activity The introduction is a statement of the subject and objectives of the experiment and presents your hypothesis. Relevant background information (appropriately referenced) is given in sufficient detail that a person unfamiliar with the topic can understand the nature of the experiment. A good introduction is clear and concise. Carbohydrates and proteins should be explained in terms of their structure and major functions in cells. Your intro should end with a brief overview of the amylase
Premium Theory Scientific method DNA
Investigation into how pH levels affect Catalase activity in potato samples – (enzymes) Introduction: The aim of this investigation is to understand and monitor the effects pH levels have on catalase activity within controlled potato samples. Catalase is a type of enzyme that is found widespread among organisms that grow in the presence of oxygen (almost all living things) its specific use is to speed up the decomposition of Hydrogen peroxide (H2O2) within cells. Hydrogen peroxide is a bi-product
Free PH Buffer solution Enzyme
escape into the air‚ and cadmium and lead may end up in the ash. As one of the most ubiquitous crops in the world‚ the potato is poised to feed the entire world. Along the way‚ scientists discovered that the popular staple of many people’s diets may also have potential to help power it as well. The potato is starchy‚ tuberous crop from the perennial nightshade . The word "potato" may refer either to the plant itself
Premium Battery Electric current Electric charge