I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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Enzyme Catalysis Maltose sugar is broken apart by maltase enzyme Substrate are molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast
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Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level‚ a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory
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Pre-Feasibility Study Potato Flakes & Flour 1 TABLE OF CONTENTS Page 1. 2. 3. 4. 5. 6. 7 8. 9. 10. 11. Objective Products Process Global Export Markets Global Import Markets Local Market Target Markets Raw Material Plant & Equipments Conclusion Role of PAMCO 3 3 3 4 5 6 7 8 9 10 10 2 OBJECTIVE: • To provide marketable and value added dehydrated potato products from raw potatoes for the use of food processing industry in Pakistan and abroad as well as end products for the consumer
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Enzymes Abstract: The following 2 labs experimented the more enzymes and substrates added to the concentration will effect the reaction rate. Our second lab‚ we tested enzyme and substrate concentrations to determine the increase of temperature and inhibitor. The enzyme source used in both labs was peroxide‚ guaiacol is used as a substrate for peroxide. We used Guaiacol‚ turnip extract‚ peroxide and distilled water for enzyme and substrate concentration. In the second lab we used the same substances
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The potato is a starchy‚ tuberous crop from the perennial Solanum tuberosum of the Solanaceae family (also known as the nightshades). The word may refer to the plant itself as well as the edible tuber. In the region of the Andes‚ there are some other closely related cultivated potato species. Potatoes were introduced outside the Andes region four centuries ago‚ and have become an integral part of much of the world’s cuisine. It is the world’s fourth-largest food crop‚ following rice‚ wheat and maize
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Enzyme Lab Write-Up Purpose: To observe how an enzyme affect the speed of chemical reaction. To describe how the concentration of an enzyme affects its ability to work. Hypothesis: Depending on the concentration of the catalase which the disk is soaked in‚ it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas. Prediction: Since the rate of reaction can be lowered by the addition of catalysis such as an enzyme. Moreover to
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2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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Research Project: Potato The potato originated in the South American Andes‚ but its heartland of wild genetic diversity reaches from Venezuela‚ Colombia‚ Ecuador‚ Peru‚ Bolivia‚ Argentina‚ Chile‚ Uruguay‚ Paraguay‚ southern Brazil‚ northward into Central America‚ Mexico‚ and the southwestern United States. Potato was first cultivated between 3 and 7 thousand years ago‚ some scientists believe they may have grown wild in the region as long as 13‚000 years ago. Potato is the third most important
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