solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic‚ the catecholase was less effective. Also‚ when there was a higher concentration of potato juice and a lower concentration of phosphate buffer‚ absorbance of the enzyme increased. Introduction According to Edmund J. Stellwag‚ in his article "Enzyme"
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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volume for the last three tubes‚ leaving us a standard to compare centrifuge times/speed with. I feel that a low power spin (rpm) with a longer time frame would achieve the best results and minimise risk‚ such as cell lyses etc. “For a typical cell homogenate‚ a 10 min. spin at low speed (400-500 x g) yields a pellet consisting of unbroken tissue‚ whole cells‚ cell nuclei‚ and large debris.” (Caprette‚
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Assay of succinate dehydrogenase of after isolation of mitochondria in Cauliflower (Brassica oleracea) using differential centrifugation. Kelly M. Messick‚ Rebecca Conner Department of Biological Sciences‚ Salisbury University‚ Salisbury‚ MD‚ 21801 U.S.A Address for correspondence: Kelly M Messick Department of Biological Sciences Salisbury University Salisbury‚ MD 21801 Phone: 410-546-2060 Fax: 410-543-6433 e-mail: km96536@gulls.salisbury.edu Running title: Assay of succinate dehydrogenase
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Food Chemistry Food Chemistry 95 (2006) 180–185 www.elsevier.com/locate/foodchem Antioxidant activity and hepatoprotective potential of Phyllanthus niruri R. Harish‚ T. Shivanandappa * Department of Food Protectants and Infestation Control‚ Central Food Technological Research Institute‚ Mysore 570 020‚ India Received 5 April 2004; received in revised form 25 November 2004; accepted 25 November 2004 Abstract Antioxidant activity and hepatoprotective potential of Phyllanthus niruri
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Variables: Independent: pH‚ enzyme concentration‚ substrate concentration and enzymatic activity. Dependent: the reaction rate Control variable: temperature and amount of substrates and enzymes added. Materials: Phosphate Buffers Beaker Catechol Potato Juice Parafilm Test Tubes Procedure: To study the effect of temperature: 1. Three different test tubes where filled with 3mL of phosphate. 2. They were set in three different temperature settings. First tube was placed in an
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cerebrum‚ hypothalamus-hippocampus‚ cerebellum and pons-medulla regions of brain were immediately removed. After the collection‚ all the brain regions were washed in normal saline. Tissue homogenate preparation: 10% homogenates of different parts of brain in phosphate buffer (0.1 M‚ pH 7.4) were prepared. Tissue homogenates were stored at -200 C and used for determination of various biochemical paramaters. For affirmation of toxicity in different brain regions due to lindane‚ a change in oxidative stress
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The effect on the pH of Distilled water‚ Potato Solution and Commercial Buffer‚ when Hydrochloric acid (0.1 mol/L) and Sodium Hydroxide (0.1 mol/L) is added Mahima Mandava Mrs. Haist September 23rd‚ 2014 SBI4U1 Background Information: The pH is the measurement of how acidic or how basic a substance can be. The pH scale is used to measure how acidic or basic a living cell can be. The pH scale ranges from 0-14; 0-6 being acidic‚ 8-14 being basic and 7 being neutral. There are many factors
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investigate the concentration of enzyme as we had previously investigated the optimum temperature for catalase in the preliminary investigation. Concentration of enzyme is also fairly easy to investigate‚ as you need to only increase the amount of potato that you want to investigate at a given time. Hypothesis As the concentration of enzyme increases it will have an effect on the rate of reaction. The suggested optimum will be 10g as the higher the mass of catalase the more enzymes‚ meaning
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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