Quantifying the COX1 Gene within the Mitochondrial DNA of a Potato Introduction Respiration is a very important process for every living organism. While it is typically thought of as breathing in oxygen‚ and exhaling carbon dioxide‚ like all things‚ it must take place at the cellular level. The electron transport chain is responsible for cellular respiration. The process uses four complexes; the fourth is cytochrome c oxidase. Cytochrome C oxidase is responsible for the reduction of oxygen to water
Premium DNA Molecular biology
instructor. Observations Data Table 1: Comparison of States of Tonicity Test Tube Solute/Solvent Appearance of Potato Strip Type of Tonicity Gradient: Is the potato strip in a Hypertonic‚ Hypotonic‚ or Isotonic at the beginning of this exercise? 1 Distilled Water Bigger Hypotonic 2 10% NaClWithered Hypertonic Data Table 2: Comparison of Turgor Pressure States in Elodea Leaf Cells Solute/Solvent
Premium Buffer solution Cell wall PH
reaction. Therefore‚ it will be presumed that the result of this experiment by isolating the polyphenol oxidase within the potato extract through the oxidation of 5mM dopa solution with highly concentrated enzymatic preparation of polyphenol oxidase with a buffer as the diluent will indicate a rapid increase in the rate of the reaction as well as the amount of activity present in the potato extract. Proposing that Vmax is proportional to enzyme concentration‚ a linear relationship should be present and enzymatic
Premium Enzyme Chemical reaction Catalysis
increase the reaction. As I increase the surface area of the potato the gas given off from the reaction will increase. Therefore to sum things up‚ my hypothesis is when the Independent variable increases so will the dependant variable. Independent variable: - Surface area of potato Dependant variable: - Gas Controlled variable: - Equal size of potatoes used - Using measuring cylinder Equipment used: - Gas collecting kit - Potato - Stop watch - Test tube - Distilled water - Hydrogen
Premium Potato Oxygen Measurement
Reduced glutathione assay (GSH) Reduced glutathione was estimated by the method of Jollow et al. [15]. 1.0 ml sample of 10% homogenate was precipitated with 1.0 ml of (4%) sulfosalicylic acid. The samples were kept at 4ºC for 1 hr and then centrifuged at 1200 × g for 20 min at 4ºC. The total volume of 3.0 ml assay mixture composed of 0.1 ml filtered aliquot‚ 2.7 ml phosphate buffer (0.1 M‚ pH 7.4) and 0.2 ml DTNB (5‚5-dithiobis-2-nitrobenzoic acid)‚ (100 mM). The yellow color of the mixture was developed
Premium Ethanol Chemistry Acetic acid
The basis of this technique is the spectrophotometric measurement of the purple color produced via the reaction of thiobarbituric acid (TBA) and MDA. Concisely‚ half a milliliter of hippocampal homogenate was mixed with 2.5 ml of trichloroacetic acid (TCA) (10% w/v) solution and the mixture was boiled in a water bath for 15 min. The samples were cooled to room temperature and the absorbance at 532 nm was measured with respect to the blank solution
Premium Acetic acid Western blot Swimming pool
solution in each of the test tubes‚ respectively 23‚ 22‚ 20‚ 16‚ 8 and 0 drops of pH 7 buffer were added. 30 drops of potato juice containing the catechol oxidase enzyme were then added to the solution‚ and then timed for 5 minutes at room temperature. The pH treatment only required 5 test tubes‚ with 3mL of matching buffer (pH4‚ pH6‚ pH7‚ pH8‚ and pH10) in the appropriate tube. Following this‚ 10 drops of both potato juice and catechol are added to the tubes‚ and then timed for 5 minutes at room temperature
Premium Enzyme Chemical reaction Metabolism
4 Fe2+ -cyt c + 8H+ + O2 4 Fe3+ -cyt c + 2H2O + 4H+ [out] Cytochrome c oxidase locates to the inner membrane which separates the mitochondrial matrix from the intermembrane space. However‚ Potato tubes can be used as the source of mitochondria‚ where this enzyme is located and active. We used potato in this lab instead of using other things because we can easily abstracts cytochrome c from it. Cytochrome c oxidize is very important in human organisms.
Premium Cellular respiration Adenosine triphosphate Metabolism
Guidelines for writing laboratory reports Laboratory reports should be written according to the format below (failure to do so will result in marks being deducted): Formatting Font Type: Times Roman Font size: 12 Spacing: 1.5‚ justified Pages : 5 (minimum) - 10 (maximum) [pages must be numbered] Title page You are required to use the lab report submission page available on the LMS and are to include these details: lab no.‚ title of experiment‚ students’ names and ID‚ date of experiment as well
Premium PH Buffer solution Cell
above or below a certain value may cause a cell distress or may even be fatal. To help maintain relatively constant pH levels‚ living systems use buffers. A buffer is a combination of a weak acid and a weak base that function together to minimize changes in the pH of a solution. In today’s lab‚ we will learn how to measure pH and demonstrate how buffers work. II) Procedure A) Carbohydrates The basic building blocks for carbohydrates are sugars‚ also called monosaccharides. These molecules are often
Premium PH