Plumbago zeylanica L. belonging to the family Plumbaginaceae is an important plant of medicinal value. P. zeylanica is greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly distributed
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1. Introduction Plumbago zeylanica L. belonging to the family Plumbaginaceae is an important plant of medicinal value. P. zeylanica is greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly
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to the numbered label (test tube 1 had 1 drop of catechol‚ test tube 16 had 16 drops‚ etc.). After these solutions were mixed‚ each tube was covered with Parafilm and inverted 3-4 times. The film was removed from the tubes and 30 drops of diluted potato juice was added to each‚ covered with Parafilm again‚ inverted 3-4 times‚ and allowed to sit at room temperature uncovered. The tubes were observed and the intensity of the colors in each tube was recorded. Results: Table1: Effect of Temperature
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Fractionation: takes cells apart and separates the major organelles and other subcellular structures from one another. * Tissue cells are the first one to be homogenate or broken apart. * Plasma membranes are broken up so that there internal contents spill out and mix together and this is called homogenate. * Homogenate is in spun in a higher rate of speed in a process called centrifugation. And that speed can vary that why it’s called differential centrifugation. * If we spin
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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For this type of experiment‚ we would generally see a decreasing trend for the total activity‚ total amount of protein‚ and percent yield and an increasing trend for specific activity and fold purification. These trends come about naturally when performing multiple purification steps. To determine the success of each purification step‚ the more important factors to look at are the total activity units‚ total protein amount‚ and percent yield. Looking at our purification table (table 7) our purifications
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Potato Lab Report: Effects of Sucrose Solutions on Potato Cells and Water Potential Abstract: Osmosis is a process by which molecules of a solvent tend to pass through a semipermeable membrane from a less concentrated solution into a more concentrated one‚ thus equalizing the concentrations on each side of the membrane. In this experiment‚ we learn about Osmosis and Diffusion through potato cores in different concentration of sucrose
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Plant Physiol. (1 996) 1 1 1 : 275-283 Sugar Accumulation in Grape Berries Cloning of Two Putative Vacuolar lnvertase cDNAs and Their Expression in Grapevine Tissues Christopher Davies* and Simon P. Robinson Cooperative Research Centre for Viticulture‚ P.O. Box 145‚ Glen Osmond‚ South Australia‚ Australia‚ and Commonwealth Scientific and industrial Research Organization‚ Division of Horticulture‚ G.P.O. Box 350‚ Adelaide‚ South Australia 5001‚ Australia The growth pattern of developing
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Independent Concentration of hydrogen peroxide Dependent Volume of oxygen gas evolved Control Amount of liver used Location of liver sample obtained from the liver Type of liver used Possible enzymes transferred from hands during making liver homogenate Deterioration of liver Endpoint identification Volume of hydrogen peroxide added Reading on the syringe Room temperature 2. Hypothesis The research question examines how different temperatures would affect the activity of catalase.
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