water and oxygen per second. Liver and other living tissues contain the enzyme catalase. Hydrogen peroxide‚ which is a harmful by-product of the process of cellular respiration is broken down if it builds up in concentration in the cells. If we use potato or other tissue containing this enzyme‚ we can use this to measure the relative influence of varying different factors on the activity of enzymes in living tissue‚ the factor I will be investigating in my coursework is the activity of pH. Aim The
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2.6 Kinetic studies of prepared complexes The integral method of Coats–Redfern equation[19‚21‚27‚38] was used for determining the kinetic parameters of the decompositions process for the investigated metal complexes according to following equation: log[log(w_∞/(w_∞-w))⁄T^2 ]〖=log[AR/〖∅E〗^* (1-2RT⁄E^≠ )]〗-E^≠/2.303R 1/T (4) Where w_∞ is the mass loss at the accomplishment of the decomposition reaction‚ w is the mass loss at temperature T‚ ∅ is the rate of heating and R is
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experiments at the same time in the same room etc. You will need to monitor this using a thermometer. 2) pH: c. Independent: A range of pH should be used to establish the bell-shaped curve. Buffers should be used to do this. d. Control: You will need to use the same buffer (appropriate to the optimum pH of the enzyme used) for every sample. 3) Substrate concentration: e. Independent: Serial dilutions‚ or % concentrations (unless you are doing Chemistry‚ wherein you
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Title: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Aim To design and conduct an experiment to demonstrate the presence of enzyme activity in the preparation provided. To examine the effect of the inhibitors provided. To test whether the other phenolic substrates provided can be oxidized by the enzyme preparation. To test for the presence of peroxidase activity in the enzyme preparation. To test the effect of the inhibitor provided on peroxidase activity
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to the numbered label (test tube 1 had 1 drop of catechol‚ test tube 16 had 16 drops‚ etc.). After these solutions were mixed‚ each tube was covered with Parafilm and inverted 3-4 times. The film was removed from the tubes and 30 drops of diluted potato juice was added to each‚ covered with Parafilm again‚ inverted 3-4 times‚ and allowed to sit at room temperature uncovered. The tubes were observed and the intensity of the colors in each tube was recorded. Results: Table1: Effect of Temperature
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Fractionation: takes cells apart and separates the major organelles and other subcellular structures from one another. * Tissue cells are the first one to be homogenate or broken apart. * Plasma membranes are broken up so that there internal contents spill out and mix together and this is called homogenate. * Homogenate is in spun in a higher rate of speed in a process called centrifugation. And that speed can vary that why it’s called differential centrifugation. * If we spin
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1. Introduction Plumbago zeylanica L. (Plumbaginaceae) is an important medicinal plant greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly distributed over Oman‚ Yemen and the Southwestern
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Plumbago zeylanica L. belonging to the family Plumbaginaceae is an important plant of medicinal value. P. zeylanica is greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly distributed
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1. Introduction Plumbago zeylanica L. belonging to the family Plumbaginaceae is an important plant of medicinal value. P. zeylanica is greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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