Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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Potato Lab Report: Effects of Sucrose Solutions on Potato Cells and Water Potential Abstract: Osmosis is a process by which molecules of a solvent tend to pass through a semipermeable membrane from a less concentrated solution into a more concentrated one‚ thus equalizing the concentrations on each side of the membrane. In this experiment‚ we learn about Osmosis and Diffusion through potato cores in different concentration of sucrose
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Plant Physiol. (1 996) 1 1 1 : 275-283 Sugar Accumulation in Grape Berries Cloning of Two Putative Vacuolar lnvertase cDNAs and Their Expression in Grapevine Tissues Christopher Davies* and Simon P. Robinson Cooperative Research Centre for Viticulture‚ P.O. Box 145‚ Glen Osmond‚ South Australia‚ Australia‚ and Commonwealth Scientific and industrial Research Organization‚ Division of Horticulture‚ G.P.O. Box 350‚ Adelaide‚ South Australia 5001‚ Australia The growth pattern of developing
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For this type of experiment‚ we would generally see a decreasing trend for the total activity‚ total amount of protein‚ and percent yield and an increasing trend for specific activity and fold purification. These trends come about naturally when performing multiple purification steps. To determine the success of each purification step‚ the more important factors to look at are the total activity units‚ total protein amount‚ and percent yield. Looking at our purification table (table 7) our purifications
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Independent Concentration of hydrogen peroxide Dependent Volume of oxygen gas evolved Control Amount of liver used Location of liver sample obtained from the liver Type of liver used Possible enzymes transferred from hands during making liver homogenate Deterioration of liver Endpoint identification Volume of hydrogen peroxide added Reading on the syringe Room temperature 2. Hypothesis The research question examines how different temperatures would affect the activity of catalase.
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from 2 to 10 mice in each experimental group were put together to produce BAL‚ total lung homogenate‚ CD11b antigen-assorting cells as well as CD11c antigen-assorting cells. The samples from the mice were obtained and cultured to be able to determine the colony forming units or bacterial load. 4) How is antigen presentation measured in this study? Antigen presentation was measured by preparing homogenates from post-washed lungs of naive mice. CD11c affinity-assorting cells were prepared the same
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The materials needed for this experiment were four medium sized tubes‚ a spectrophotometer‚ a buffer with a pH of 5‚ H2O2‚ Peroxidase‚ and Guaiacol Dye. We as a group had four tubes labeled two‚ three‚ four‚ and five. In the tube labeled two we had a solution of 2.0 mL of H2O2 and 1.0 mL of Guaiacol Dye for a total solution of 3.0 mL. In the tube labeled three we had a solution of 4.0 mL buffer and 1.0 mL Peroxidase for a total of 5.0 mL solution. In the tube labeled four we had a solution of
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Enzyme lab report. Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize‚ bind‚ and change specific reactants. They do not change thus can catalyze the same reaction again and again. Activation energy also known as an energy barrier is the amount of energy needed in order to begin a chemical reaction. Catecholase catalyzes the reaction rate of catechol oxidation. Catechol is found beneath the skin of many plants such
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proven that pH buffer 7 is the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports my hypothesis: At pH buffer 7 the enzyme
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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