HOMOGENIZATION • Take chilled liver tissue and cut into smaller pieces. • Place liver into blender with cold isotonic solution. • Blend until you have a thick soup like substance (homogenate). • Filter the mixture to remove large debris through gauze into a tube. DIFFERENTIAL CENTRIFUGATION • Attach tubes of homogenate to the refrigerated Centrifuge and turn on to spin the tubes and separate the organelles in order of mass. As per fig 1. • Centrifuge @ 600 x g for 5 minutes to separate Nuclei
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concentration‚ the affinity of the enzyme for the substrate (how well the enzyme binds to the substrate)‚ concentrations of cofactors and coenzymes‚ pH and temperature. In this experiment‚ we will be measuring enzyme activity in samples of muscle homogenate (skeletal muscle that has been broken up) by measuring how much light is absorbed by NADH in the above equation‚ which will be related to how much NADH is present. We will then determine LDH activity by using the formula: ([ A/min]/6.22) x 78‚780
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reaction. Material • Potato • 250mL Beaker • Four 100mm test tubes • Mortar and Pestle • Refrigerator • Hydrogen Peroxide • 7.0 pH Phosphate buffer solution • Thermometer Methods The potato was crushed with a mortar and pestle to make a slurry (the catalase) and poured it into a 250 ml beaker. Four 100 mm test tubes (marked at every cm) were filled from the beaker with a mixture of the catalase and a 7.0 pH phosphate buffer solution to the 1 cm mark
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Effect of Temperature on Peroxidase Ability to Break Down H2O2 By: Rodneika Crutcher Abstract Temperature affects the ability of peroxidase to break down hydrogen peroxide. In this experiment our professor extracted peroxidase from potato tissue. In order to determine how temperature affects peroxidase we created solutions and measured their absorbance levels after water bath treatments. The more absorbent the solution was the less hydrogen peroxide there was in the solution. This means the peroxidase
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Writing of Lab Reports __________________________________________________________________________ W hy should I bother writing lab reports in the correct way?” The Foundation Programme is designed to prepare you for undergraduate studies at UTAR which will require the writing of lab reports all years generally. At the end of your third year‚ you may have an opportunity to work on scientific projects which will culminate in an official scientific report. Depending on the quality of your report
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tubes 5a-5g 2. Lay the test tubes against a metric ruler and mark lines indicating 4cm‚ 5cm‚ 6cm‚ at the bottom of each test tube. 3. Put your test tubes to the location of the phosphate buffer series and fill each tube according to the following direction. 4. Continue your work area and add 1cm of potato extract obtaining catechol oxidase to each of the seven tubes. 5. Add 1% catechol to each of the 7 test tubes‚ bringing the total volume to the 6c mark. Agitate the contents of the tubes using
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conditions in potato juice extract Introduction The presence of quinones prevents infection of microorganisms because it is toxic to them (Danyk‚ H‚ 2013). Quinones are produced by the oxidation of phenolic compound of catechol. Enzymes are used to speed up chemical reactions in cells (Danyk‚ H‚ 2013). The enzyme catechol oxidase is used to speed up the production of benzoquinone which is to help in infection prevention. In this study catechol oxidase was combined with potato juice extract
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Board(IndependentVariable) | 1 Sharp Knife(Independent Variable) | 1 Aluminum Foil 6 x 6 cm(Dependent Variable) | 1 Pipet Empty‚ Short Stem(Dependent Variable) | 1 Pipet‚ Graduated Jumbo (5 mL)(Dependent Variable) | 1 Distilled Water(Independent Variable) | 1 Potato(Independent Variable) | 2 Beaker‚ 50 mL Plastic(Dependent Variable) | 1 Test Tube(9)‚ 13 x 100 mm(Dependent Variable) | 1 Benedict’s Reagent - 15 mL in Dropper Bottle(Dependent Variable) | 1 Stove or Microwave(Independent Variable) | 1 Aluminum
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activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9. Filter potato extract 10. Place extract into a 100ml graduated cylinder 11. Add cold distilled
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Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic
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